Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 23 October 1992:
Vol. 258. no. 5082, pp. 656 - 659
DOI: 10.1126/science.1329207
Prev | Table of Contents | Next

Articles

Science, Vol 258, Issue 5082, 656-659
Copyright © 1992 by American Association for the Advancement of Science

articles

Flow-dependent cytosolic acidification of vascular endothelial cells

RC Ziegelstein, L Cheng, and MC Capogrossi

Laboratory of Cardiovascular Science, National Institute on Aging, National Institutes of Health, Baltimore, MD 21224.

Hemodynamic shear stress affects endothelial cell structure and function, but little is known about the signal transduction mechanisms involved in these processes. The effect of laminar shear stress on cytosolic pH (pHi) was examined in rat aortic endothelial cells cultured in glass capillary tubes. Shear stress forces led to a rapid decrease in pHi (maximal effect 0.09 pH unit at 13.4 dynes per square centimeter). Removal of specific ions or addition of exchange inhibitors suggests that in vascular endothelial cells shear stress forces activate both an alkali extruder, sodium ion-independent chloride-bicarbonate ion exchange, and an acid extruder, sodium-hydrogen ion exchange; the net effect in physiologic buffer with the bicarbonate ion is a decrease in pHi.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transporters involved in regulation of intracellular pH in primary cultured rat brain endothelial cells.
C. J. Taylor, P. A. Nicola, S. Wang, M. A. Barrand, and S. B. Hladky (2006)
J. Physiol. 576, 769-785
   Abstract »    Full Text »    PDF »
Vacuolar-type H+-ATPases at the plasma membrane regulate pH and cell migration in microvascular endothelial cells.
J. D. Rojas, S. R. Sennoune, D. Maiti, K. Bakunts, M. Reuveni, S. C. Sanka, G. M. Martinez, E. A. Seftor, C. J. Meininger, G. Wu, et al. (2006)
Am J Physiol Heart Circ Physiol 291, H1147-H1157
   Abstract »    Full Text »    PDF »
Axl receptor activation mediates laminar shear stress anti-apoptotic effects in human endothelial cells.
D. D'Arcangelo, V. Ambrosino, M. Giannuzzo, C. Gaetano, and M. C. Capogrossi (2006)
Cardiovasc Res 71, 754-763
   Abstract »    Full Text »    PDF »
Control of neutrophil pseudopods by fluid shear: role of Rho family GTPases.
A. Makino, M. Glogauer, G. M. Bokoch, S. Chien, and G. W. Schmid-Schonbein (2005)
Am J Physiol Cell Physiol 288, C863-C871
   Abstract »    Full Text »    PDF »
Both Metabolic Inhibition and Mitochondrial KATP Channel Opening Are Myoprotective and Initiate a Compensatory Sarcolemmal Outward Membrane Current.
H. Irie, J. Gao, G. R. Gaudette, I. S. Cohen, R. T. Mathias, A. E. Saltman, and I. B. Krukenkamp (2003)
Circulation 108, II-341-347
   Abstract »    Full Text »    PDF »
Differential effects of pulsatile versus steady flow on coronary endothelial membrane potential.
W.-P. Qiu, Q. Hu, N. Paolocci, R. C. Ziegelstein, and D. A. Kass (2003)
Am J Physiol Heart Circ Physiol 285, H341-H346
   Abstract »    Full Text »    PDF »
Depletion of Intracellular Ca2+ Stores Sensitizes the Flow-Induced Ca2+ Influx in Rat Endothelial Cells.
H.-Y. Kwan, P.-C. Leung, Y. Huang, and X. Yao (2003)
Circ. Res. 92, 286-292
   Abstract »    Full Text »    PDF »
Acidification Prevents Endothelial Cell Apoptosis by Axl Activation.
D. D'Arcangelo, C. Gaetano, and M. C. Capogrossi (2002)
Circ. Res. 91 , e4-e12
   Abstract »    Full Text »    PDF »
Propionate-induced relaxation in rat mesenteric arteries: a role for endothelium-derived hyperpolarising factor.
G Knock, D Psaroudakis, S Abbot, and P I Aaronson (2002)
J. Physiol. 538, 879-890
   Abstract »    Full Text »    PDF »
Na+/H+ exchange inhibition prevents endothelial dysfunction after I/R injury.
R. J. Gumina, J. Moore, P. Schelling, N. Beier, and G. J. Gross (2001)
Am J Physiol Heart Circ Physiol 281, H1260-H1266
   Abstract »    Full Text »    PDF »
Role of endothelial [Ca2+]i in activation of eNOS in pressurized arterioles by agonists and wall shear stress.
Z. Ungvari, D. Sun, A. Huang, G. Kaley, and A. Koller (2001)
Am J Physiol Heart Circ Physiol 281, H606-H612
   Abstract »    Full Text »    PDF »
Cellular Responses to Mechanical Stress: Invited Review: Effects of flow on vascular endothelial intracellular calcium signaling of rat aortas ex vivo.
C. J. Jen, S.-J. Jhiang, and H.-I. Chen (2000)
J Appl Physiol 89, 1657-1662
   Abstract »    Full Text »    PDF »
In vitro system to study realistic pulsatile flow and stretch signaling in cultured vascular cells.
X. Peng, F. A. Recchia, B. J. Byrne, I. S. Wittstein, R. C. Ziegelstein, and D. A. Kass (2000)
Am J Physiol Cell Physiol 279, C797-C805
   Abstract »    Full Text »    PDF »
Opposite Effects of Pressurized Steady Versus Pulsatile Perfusion on Vascular Endothelial Cell Cytosolic pH : Role of Tyrosine Kinase and Mitogen-Activated Protein Kinase Signaling.
I. S. Wittstein, W. Qiu, R. C. Ziegelstein, Q. Hu, and D. A. Kass (2000)
Circ. Res. 86, 1230-1236
   Abstract »    Full Text »    PDF »
Acidosis Inhibits Endothelial Cell Apoptosis and Function and Induces Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor Expression.
D. D’Arcangelo, F. Facchiano, L. M. Barlucchi, G. Melillo, B. Illi, L. Testolin, C. Gaetano, and M. C. Capogrossi (2000)
Circ. Res. 86, 312-318
   Abstract »    Full Text »    PDF »
Two distinct HCO-3-dependent H+ efflux pathways in human vascular endothelial cells.
B. Sun, R. D. Vaughan-Jones, and J.-I. Kambayashi (1999)
Am J Physiol Heart Circ Physiol 277, H28-H32
   Abstract »    Full Text »    PDF »
Hydrogen Peroxide Decreases pHi in Human Aortic Endothelial Cells by Inhibiting Na+/H+ Exchange.
Q. Hu, Y. Xia, S. Corda, J. L. Zweier, and R. C. Ziegelstein (1998)
Circ. Res. 83, 644-651
   Abstract »    Full Text »    PDF »
Cytosolic Alkalinization of Vascular Endothelial Cells Produced by an Abrupt Reduction in Fluid Shear Stress.
R. C. Ziegelstein, P. S. Blank, L. Cheng, and M. C. Capogrossi (1998)
Circ. Res. 82, 803-809
   Abstract »    Full Text »    PDF »
Regulation of endothelin-1 gene expression by cell shape and the microfilament network in vascular endothelium.
A. M. Malek, I. W. Lee, S. L. Alper, and S. Izumo (1997)
Am J Physiol Cell Physiol 273, C1764-C1774
   Abstract »    Full Text »    PDF »
The Effects of Hypoxia on pHi in Porcine Coronary Artery Endothelium and Smooth Muscle: A Novel Method for Measurements in Endothelial Cells In Situ.
R. A. Foy, S. Shimizu, and R. J. Paul (1997)
Circ. Res. 80, 21-27
   Abstract »    Full Text »
Intracellular pH and Tyrosine Phosphorylation but Not Calcium Determine Shear Stress–Induced Nitric Oxide Production in Native Endothelial Cells.
K. Ayajiki, M. Kindermann, M. Hecker, I. Fleming, and R. Busse (1996)
Circ. Res. 78, 750-758
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)