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Science 9 October 1992:
Vol. 258. no. 5080, pp. 310 - 313
DOI: 10.1126/science.1357749
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Articles

Science, Vol 258, Issue 5080, 310-313
Copyright © 1992 by American Association for the Advancement of Science

articles

Calcium channels coupled to glutamate release identified by omega-Aga-IVA

TJ Turner, ME Adams, and K Dunlap

Department of Physiology, Tufts University School of Medicine, Boston, MA 02111.

Presynaptic calcium channels are crucial elements of neuronal excitation-secretion coupling. In mammalian brain, they have been difficult to characterize because most presynaptic terminals are too small to probe with electrodes, and available pharmacological tools such as dihydropyridines and omega-conotoxin are largely ineffective. Subsecond measurements of synaptosomal glutamate release have now been used to assess presynaptic calcium channel activity in order to study the action of peptide toxins from the venom of the funnel web spider Agelenopsis aperta, which is known to inhibit dihydropyridine and omega-conotoxin-resistant neuronal calcium currents. A presynaptic calcium channel important in glutamate release is shown to be omega-Aga-IVA sensitive and omega-conotoxin resistant.


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