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Science 9 October 1992:
Vol. 258. no. 5080, pp. 287 - 292
DOI: 10.1126/science.258.5080.287
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Articles

Regulation of the Amount of Starch in Plant Tissues by ADP Glucose Pyrophosphorylase

David M. Stark 1, Kurt P. Timmerman 1, Gerard F. Barry 1, Jack Preiss 2, and Ganesh M. Kishore 1

1 Monsanto Company, 700 Chesterfield Village Parkway, St. Louis, MO 63198
2 Department of Biochemistry, Michigan State University, East Lansing, MI 48824

Starch, a major storage metabolite in plants, positively affects the agricultural yield of a number of crops. Its biosynthetic reactions use adenosine diphosphate glucose (ADPGlc) as a substrate; ADPGlc pyrophosphorylase, the enzyme involved in ADPGlc formation, is regulated by allosteric effectors. Evidence that this plastidial enzyme catalyzes a rate-limiting reaction in starch biosynthesis was derived by expression in plants of a gene that encodes a regulatory variant of this enzyme. Allosteric regulation was demonstrated to be the major physiological mechanism that controls starch biosynthesis. Thus, plant and bacterial systems for starch and glycogen biosynthesis are similar and distinct from yeast and mammalian systems, wherein glycogen synthase has been demonstrated to be the rate-limiting regulatory step.

Submitted on April 29, 1992
Accepted on July 31, 1992


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