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Science 9 October 1992:
Vol. 258. no. 5080, pp. 269 - 271
DOI: 10.1126/science.1411525
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Articles

Science, Vol 258, Issue 5080, 269-271
Copyright © 1992 by American Association for the Advancement of Science

articles

X-ray laser microscopy of rat sperm nuclei

LB Da Silva, JE Trebes, R Balhorn, S Mrowka, E Anderson, DT Attwood, TW Barbee Jr, J Brase, M Corzett, J Gray, and al. et

Department of Physics, University of California, Berkeley 94720.

The development of high brightness and short pulse width (< 200 picoseconds) x-ray lasers now offers biologists the possibility of high-resolution imaging of specimens in an aqueous environment without the blurring effects associated with natural motions and chemical erosion. As a step toward developing the capabilities of this type of x-ray microscopy, a tantalum x-ray laser at 44.83 angstrom wavelength was used together with an x-ray zone plate lens to image both unlabeled and selectively gold-labeled dried rat sperm nuclei. The observed images show approximately 500 angstrom features, illustrate the importance of x-ray microscopy in determining chemical composition, and provide information about the uniformity of sperm chromatin organization and the extent of sperm chromatin hydration.


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