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Science 25 September 1992:
Vol. 257. no. 5078, pp. 1951 - 1955
DOI: 10.1126/science.1290524
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Articles

Science, Vol 257, Issue 5078, 1951-1955
Copyright © 1992 by American Association for the Advancement of Science

articles

Sequence discrimination by alternatively spliced isoforms of a DNA binding zinc finger domain

JA Gogos, T Hsu, J Bolton, and FC Kafatos

Department of Cellular and Developmental Biology, Harvard University, Cambridge, MA 02138.

Two major developmentally regulated isoforms of the Drosophila chorion transcription factor CF2 differ by an extra zinc finger within the DNA binding domain. The preferred DNA binding sites were determined and are distinguished by an internal duplication of TAT in the site recognized by the isoform with the extra finger. The results are consistent with modular interactions between zinc fingers and trinucleotides and also suggest rules for recognition of AT-rich DNA sites by zinc finger proteins. The results show how modular finger interactions with trinucleotides can be used, in conjunction with alternative splicing, to alter the binding specificity and increase the spectrum of sites recognized by a DNA binding domain. Thus, CF2 may potentially regulate distinct sets of target genes during development.


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