Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 25 September 1992:
Vol. 257. no. 5078, pp. 1944 - 1946
DOI: 10.1126/science.1411511
Prev | Table of Contents | Next

Articles

Science, Vol 257, Issue 5078, 1944-1946
Copyright © 1992 by American Association for the Advancement of Science

articles

Actin filament dynamics in living glial cells imaged by atomic force microscopy

E Henderson, PG Haydon, and DS Sakaguchi

Department of Zoology and Genetics, Iowa State University, Ames 50011.

Observation of filamentous actin (F-actin) in living cells is currently limited to the resolution of the light microscope. Higher resolution procedures require sample fixation and preclude dynamic studies. The atomic force microscope (AFM) can image and manipulate samples at very high, sometimes atomic resolution by scanning a fine tip over the surface of interest and detecting physical interactions between the tip and sample. This study demonstrates that F-actin can be readily resolved in living cells with the AFM and that the dynamic properties of F-actin are easily observed.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Label-Free Protein and Pathogen Detection Using the Atomic Force Microscope.
J. L. Huff, M. P. Lynch, S. Nettikadan, J. C. Johnson, S. Vengasandra, and E. Henderson (2004)
J Biomol Screen 9, 491-497
   Abstract »    PDF »
Morphogenesis of Bacillus Spore Surfaces.
V. G. R. Chada, E. A. Sanstad, R. Wang, and A. Driks (2003)
J. Bacteriol. 185, 6255-6261
   Abstract »    Full Text »    PDF »
Physical Morphology and Surface Properties of Unsaturated Pseudomonas putida Biofilms.
I. D. Auerbach, C. Sorensen, H. G. Hansma, and P. A. Holden (2000)
J. Bacteriol. 182, 3809-3815
   Abstract »    Full Text »
Pushing, pulling, dragging, and vibrating renal epithelia by using atomic force microscopy.
R. M. Henderson and H. Oberleithner (2000)
Am J Physiol Renal Physiol 278, F689-F701
   Abstract »    Full Text »    PDF »
Continuous detection of extracellular ATP on living cells by using atomic force microscopy.
S. W. Schneider, M. E. Egan, B. P. Jena, W. B. Guggino, H. Oberleithner, and J. P. Geibel (1999)
PNAS 96, 12180-12185
   Abstract »    Full Text »    PDF »
Direct Probing of the Surface Ultrastructure and Molecular Interactions of Dormant and Germinating Spores of Phanerochaete chrysosporium.
Y. F. Dufrêne, C. J. P. Boonaert, P. A. Gerin, M. Asther, and P. G. Rouxhet (1999)
J. Bacteriol. 181, 5350-5354
   Abstract »    Full Text »
Probing Nanometer Structures with Atomic Force Microscopy.
Z. Shao (1999)
Physiology 14, 142-149
   Abstract »    Full Text »    PDF »
Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy.
C. Rotsch, K. Jacobson, and M. Radmacher (1999)
PNAS 96, 921-926
   Abstract »    Full Text »    PDF »
Surface dynamics in living acinar cells imaged by atomic force microscopy: Identification of plasma membrane structures involved in exocytosis.
S. W. Schneider, K. C. Sritharan, J. P. Geibel, H. Oberleithner, and B. P. Jena (1997)
PNAS 94, 316-321
   Abstract »    Full Text »    PDF »
Fibrous Mini-Collagens in Hydra Nematocysts.
T. W. Holstein, M. Benoit, G. v. Herder, C. N. David, G. Wanner, and H. E. Gaub (1994)
Science 265, 402-404
   Abstract »    PDF »
Imaging subcellular structures of rat mammary carcinoma cells by scanning force microscopy.
L. Pietrasanta, A Schaper, and T. Jovin (1994)
J. Cell Sci. 107, 2427-2437
   Abstract »    PDF »
Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy.
J. Hoh and C. Schoenenberger (1994)
J. Cell Sci. 107, 1105-1114
   Abstract »    PDF »
Three-dimensional imaging of living neurons and glia with the atomic force microscope.
V Parpura, P. Haydon, and E Henderson (1993)
J. Cell Sci. 104, 427-432
   Abstract »    PDF »
From molecules to cells: imaging soft samples with the atomic force microscope.
M Radmacher, R. Tillamnn, M Fritz, and H. Gaub (1992)
Science 257, 1900-1905
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)