Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 11 September 1992:
Vol. 257. no. 5076, pp. 1557 - 1559
DOI: 10.1126/science.1387971
Prev | Table of Contents | Next

Articles

Science, Vol 257, Issue 5076, 1557-1559
Copyright © 1992 by American Association for the Advancement of Science

articles

Regulation of dynein-driven microtubule sliding by the radial spokes in flagella

EF Smith and WS Sale

Department of Anatomy and Cell Biology, Emory University School of Medicine, Atlanta, GA 30322.

The regulation of microtubule sliding in flagellar axonemes was studied with the use of Chlamydomonas mutants and in vitro assays. Microtubule sliding velocities were diminished in axonemes from mutant cells missing radial spoke structures but could be restored upon reconstitution with dynein from axonemes with wild-type radial spokes. These experiments demonstrate that the radial spokes activate dynein's microtubule sliding activity.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Regulation of dynein-driven microtubule sliding by the axonemal protein kinase CK1 in Chlamydomonas flagella.
A. Gokhale, M. Wirschell, and W. S. Sale (2009)
J. Cell Biol. 186, 817-824
   Abstract »    Full Text »    PDF »
IC138 Defines a Subdomain at the Base of the I1 Dynein That Regulates Microtubule Sliding and Flagellar Motility.
R. Bower, K. VanderWaal, E. O'Toole, L. Fox, C. Perrone, J. Mueller, M. Wirschell, R. Kamiya, W. S. Sale, and M. E. Porter (2009)
Mol. Biol. Cell 20, 3055-3063
   Abstract »    Full Text »    PDF »
Homologues of Radial Spoke Head Proteins Interact with Ca2+/Calmodulin in Tetrahymena Cilia.
H. Ueno, Y. Iwataki, and O. Numata (2006)
J. Biochem. 140, 525-533
   Abstract »    Full Text »    PDF »
Conserved and specific functions of axoneme components in trypanosome motility.
C. Branche, L. Kohl, G. Toutirais, J. Buisson, J. Cosson, and P. Bastin (2006)
J. Cell Sci. 119, 3443-3455
   Abstract »    Full Text »    PDF »
Disruption of the A-Kinase Anchoring Domain in Flagellar Radial Spoke Protein 3 Results in Unregulated Axonemal cAMP-dependent Protein Kinase Activity and Abnormal Flagellar Motility.
A. R. Gaillard, L. A. Fox, J. M. Rhea, B. Craige, and W. S. Sale (2006)
Mol. Biol. Cell 17, 2626-2635
   Abstract »    Full Text »    PDF »
Flagellar Motility Contributes to Cytokinesis in Trypanosoma brucei and Is Modulated by an Evolutionarily Conserved Dynein Regulatory System..
K. S. Ralston, A. G. Lerner, D. R. Diener, and K. L. Hill (2006)
Eukaryot. Cell 5, 696-711
   Abstract »    Full Text »    PDF »
Radial spoke proteins of Chlamydomonas flagella.
P. Yang, D. R. Diener, C. Yang, T. Kohno, G. J. Pazour, J. M. Dienes, N. S. Agrin, S. M. King, W. S. Sale, R. Kamiya, et al. (2006)
J. Cell Sci. 119, 1165-1174
   Abstract »    Full Text »    PDF »
Two Anti-radial Spoke Monoclonal Antibodies Inhibit Chlamydomonas Axonemal Motility by Different Mechanisms.
D. White, S. Aghigh, I. Magder, J. Cosson, P. Huitorel, and C. Gagnon (2005)
J. Biol. Chem. 280, 14803-14810
   Abstract »    Full Text »    PDF »
IC138 Is a WD-Repeat Dynein Intermediate Chain Required for Light Chain Assembly and Regulation of Flagellar Bending.
T. W. Hendrickson, C. A. Perrone, P. Griffin, K. Wuichet, J. Mueller, P. Yang, M. E. Porter, and W. S. Sale (2004)
Mol. Biol. Cell 15, 5431-5442
   Abstract »    Full Text »    PDF »
The LC7 Light Chains of Chlamydomonas Flagellar Dyneins Interact with Components Required for Both Motor Assembly and Regulation.
L. M. DiBella, M. Sakato, R. S. Patel-King, G. J. Pazour, and S. M. King (2004)
Mol. Biol. Cell 15, 4633-4646
   Abstract »    Full Text »    PDF »
Bend propagation drives central pair rotation in Chlamydomonas reinhardtii flagella.
D. R. Mitchell and M. Nakatsugawa (2004)
J. Cell Biol. 166, 709-715
   Abstract »    Full Text »    PDF »
Cpc1, a Chlamydomonas central pair protein with an adenylate kinase domain.
H. Zhang and D. R. Mitchell (2004)
J. Cell Sci. 117, 4179-4188
   Abstract »    Full Text »    PDF »
Flagellar Radial Spokes Contain a Ca2+-stimulated Nucleoside Diphosphate Kinase.
R. S. Patel-King, O. Gorbatyuk, S. Takebe, and S. M. King (2004)
Mol. Biol. Cell 15, 3891-3902
   Abstract »    Full Text »    PDF »
A Novel Tctex2-related Light Chain Is Required for Stability of Inner Dynein Arm I1 and Motor Function in the Chlamydomonas Flagellum.
L. M. DiBella, E. F. Smith, R. S. Patel-King, K.-i. Wakabayashi, and S. M. King (2004)
J. Biol. Chem. 279, 21666-21676
   Abstract »    Full Text »    PDF »
Flagellar Radial Spoke Protein 2 Is a Calmodulin Binding Protein Required for Motility in Chlamydomonas reinhardtii.
P. Yang, C. Yang, and W. S. Sale (2004)
Eukaryot. Cell 3, 72-81
   Abstract »    Full Text »    PDF »
A subunit of the dynein regulatory complex in Chlamydomonas is a homologue of a growth arrest-specific gene product.
G. Rupp and M. E. Porter (2003)
J. Cell Biol. 162, 47-57
   Abstract »    Full Text »    PDF »
Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis.
P. Padma, Y. Satouh, K.-i. Wakabayashi, A. Hozumi, Y. Ushimaru, R. Kamiya, and K. Inaba (2003)
Mol. Biol. Cell 14, 774-785
   Abstract »    Full Text »    PDF »
Regulation of Flagellar Dynein by Calcium and a Role for an Axonemal Calmodulin and Calmodulin-dependent Kinase.
E. F. Smith (2002)
Mol. Biol. Cell 13, 3303-3313
   Abstract »    Full Text »    PDF »
Localization of Calmodulin and Dynein Light Chain Lc8 in Flagellar Radial Spokes.
P. Yang, D. R. Diener, J. L. Rosenbaum, and W. S. Sale (2001)
J. Cell Biol. 153, 1315-1326
   Abstract »    Full Text »    PDF »
Flagellar Radial Spoke Protein 3 Is an a-Kinase Anchoring Protein (Akap).
A. R. Gaillard, D. R. Diener, J. L. Rosenbaum, and W. S. Sale (2001)
J. Cell Biol. 153, 443-448
   Abstract »    Full Text »    PDF »
The 9 + 2 Axoneme Anchors Multiple Inner Arm Dyneins and a Network of Kinases and Phosphatases That Control Motility.
M. E. Porter and W. S. Sale (2000)
J. Cell Biol. 151, 37-42
   Full Text »    PDF »
Calcium regulation of microtubule sliding in reactivated sea urchin sperm flagella.
H Bannai, M Yoshimura, K Takahashi, and C Shingyoji (2000)
J. Cell Sci. 113, 831-839
   Abstract »    PDF »
Protein phosphatases PP1 and PP2A are located in distinct positions in the Chlamydomonas flagellar axoneme.
P Yang, L Fox, R. Colbran, and W. Sale (2000)
J. Cell Sci. 113, 91-102
   Abstract »    PDF »
Characterization of a Chlamydomonas Insertional Mutant that Disrupts Flagellar Central Pair Microtubule-associated Structures.
D. R. Mitchell and W. S. Sale (1999)
J. Cell Biol. 144, 293-304
   Abstract »    Full Text »    PDF »
Molecular Cloning and Characterization of a Radial Spoke Head Protein of Sea Urchin Sperm Axonemes: Involvement of the Protein in the Regulation of Sperm Motility.
D. Gingras, D. White, J. Garin, J. Cosson, P. Huitorel, H. Zingg, C. Cibert, and C. Gagnon (1998)
Mol. Biol. Cell 9, 513-522
   Abstract »    Full Text »
The axonemal microtubules of the Chlamydomonas flagellum differ in tubulin isoform content.
K. Johnson (1998)
J. Cell Sci. 111, 313-320
   Abstract »    PDF »
Transport of a novel complex in the cytoplasmic matrix of Chlamydomonas flagella.
G. Piperno and K. Mead (1997)
PNAS 94, 4457-4462
   Abstract »    Full Text »    PDF »
Phosphoregulation of an Inner Dynein Arm Complex in Chlamydomonas reinhardtii Is Altered in Phototactic Mutant Strains.
S. J. King and S. K. Dutcher (1997)
J. Cell Biol. 136, 177-191
   Abstract »    Full Text »    PDF »
Regulation of Flagellar Dynein by Phosphorylation of a 138-kD Inner Arm Dynein Intermediate Chain.
G. Habermacher and W. S. Sale (1997)
J. Cell Biol. 136, 167-176
   Abstract »    Full Text »    PDF »
Regulation of flagellar dynein by an axonemal type-1 phosphatase in Chlamydomonas.
G Habermacher and W. Sale (1996)
J. Cell Sci. 109, 1899-1907
   Abstract »    PDF »
Confocal microscopy analysis of living Xenopus eggs and the mechanism of cortical rotation.
C. Larabell, B. Rowning, J Wells,, M Wu, and J. Gerhart (1996)
Development 122, 1281-1289
   Abstract »    PDF »
Cyclical bending movements induced locally by successive iontophoretic application of ATP to an elastase-treated flagellar axoneme.
C Shingyoji and K Takahashi (1995)
J. Cell Sci. 108, 1359-1369
   Abstract »    PDF »
Casein Kinase I Is Anchored on Axonemal Doublet Microtubules and Regulates Flagellar Dynein Phosphorylation and Activity.
P. Yang and W. S. Sale (2000)
J. Biol. Chem. 275, 18905-18912
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)