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Science 4 September 1992:
Vol. 257. no. 5075, pp. 1410 - 1412
DOI: 10.1126/science.1388286
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Articles

Science, Vol 257, Issue 5075, 1410-1412
Copyright © 1992 by American Association for the Advancement of Science

articles

Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model

G van den Engh, R Sachs, and BJ Trask

Human Genome Center, Lawrence Livermore National Laboratory, Livermore, CA 94550.

The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ sequences chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp) region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosome. achieved by hybridization to metaphase chromosomes.


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