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Science 4 September 1992:
Vol. 257. no. 5075, pp. 1392 - 1395
DOI: 10.1126/science.1529339
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Articles

Science, Vol 257, Issue 5075, 1392-1395
Copyright © 1992 by American Association for the Advancement of Science

articles

Cloning of the 62-kilodalton component of basic transcription factor BTF2

L Fischer, M Gerard, C Chalut, Y Lutz, S Humbert, M Kanno, P Chambon, and JM Egly

Laboratoire de Genetique Moleculaire des Eucaryotes, Centre National de la Recherche Scientifique, Unite 184, Faculte de Medecine, Strasbourg, France.

Cloning of the mammalian basic transcription factors serves as a major step in understanding the mechanism of transcription initiation. The 62-kilodalton component (p62) of one of these transcription factors, BTF2 was cloned and overexpressed. A monoclonal antibody to this polypeptide inhibited transcription in vitro. Immunoaffinity experiments demonstrated that the 62-kilodalton component is closely associated with the other polypeptides present in the BTF2 factor. Sequence similarity suggests that BTF2 may be the human counterpart of RNA polymerase II initiation factor b from yeast.


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Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex..
K Yokomori, A Admon, J A Goodrich, J L Chen, and R Tjian (1993)
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Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II..
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An ATP-dependent inhibitor of TBP binding to DNA..
D T Auble and S Hahn (1993)
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DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor.
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Cloning of a subunit of yeast RNA polymerase II transcription factor b and CTD kinase.
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TFIIH Interacts with the Retinoic Acid Receptor gamma and Phosphorylates Its AF-1-activating Domain through cdk7.
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TFIIH Inhibits CDK9 Phosphorylation during Human Immunodeficiency Virus Type 1 Transcription.
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