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Science 4 September 1992:
Vol. 257. no. 5075, pp. 1382 - 1384
DOI: 10.1126/science.1529337
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Articles

Science, Vol 257, Issue 5075, 1382-1384
Copyright © 1992 by American Association for the Advancement of Science

articles

The Autographa californica baculovirus genome: evidence for multiple replication origins

M Pearson, R Bjornson, G Pearson, and G Rohrmann

Department of Agricultural Chemistry, Oregon State University, Corvallis 97331.

The Autographa californica multinucleocapsid nuclear polyhedrosis virus (AcMNPV), which is used for the overexpression of eukaryotic genes and is being engineered for possible use as a viral insecticide, has a circular, supercoiled genome of approximately 128 kilobases. Despite its widespread use, little is known about the mechanism by which AcMNPV replicates. Evidence is presented in this report that AcMNPV origins of DNA replication are repeated sequences each containing several closely related imperfect palindromes that are present in six regions distributed around the genome. Although AcMNPV infection-dependent plasmid replication was initiated by a single complete palindrome, the amount of replication was substantially increased in plasmids containing six or eight palindromes.


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