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Science 28 August 1992: Vol. 257. no. 5074, pp. 1225 - 1230 DOI: 10.1126/science.1519059
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Articles
Science, Vol 257, Issue 5074, 1225-1230
Copyright © 1992 by American Association for the Advancement of Science
Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel
M Li,
YN Jan,
and
LY Jan
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
The functional heterogeneity of potassium channels in eukaryotic cells arises not only from the multiple potassium channel genes and splice variants but also from the combinatorial mixing of different potassium channel polypeptides to form heteromultimeric channels with distinct properties. One structural element that determines the compatibility of different potassium channel polypeptides in subunit assembly has now been localized to the hydrophilic amino-terminal domain. A Drosophila Shaker B (ShB) potassium channel truncated polypeptide that contains only the hydrophilic amino-terminal domain can form a homomultimer; the minimal requirement for the homophilic interaction has been localized to a fragment of 114 amino acids. Substitution of the amino-terminal domain of a distantly related mammalian potassium channel polypeptide (DRK1) with that of ShB permits the chimeric DRK1 polypeptide to coassemble with ShB.
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278, 110-113
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- Suppression of Slow Delayed Rectifier Current by a Truncated Isoform of KvLQT1 Cloned from Normal Human Heart.
- M. Jiang, J. Tseng-Crank, and G.-N. Tseng (1997)
J. Biol. Chem.
272, 24109-24112
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- Quantitative Single-Cell-Reverse Transcription-PCR Demonstrates That A-Current Magnitude Varies as a Linear Function of shal Gene Expression in Identified Stomatogastric Neurons.
- D. J. Baro, R. M. Levini, M. T. Kim, A. R. Willms, C. C. Lanning, H. E. Rodriguez, and R. M. Harris-Warrick (1997)
J. Neurosci.
17, 6597-6610
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- A Mammalian Transient Type K+ Channel, Rat Kv1.4, Has Two Potential Domains That Could Produce Rapid Inactivation.
- S.-i. Kondoh, K. Ishii, Y. Nakamura, and N. Taira (1997)
J. Biol. Chem.
272, 19333-19338
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- Extracellular Interaction of the Voltage-dependent Ca2+ Channel alpha 2delta and alpha 1 Subunits.
- C. A. Gurnett, R. Felix, and K. P. Campbell (1997)
J. Biol. Chem.
272, 18508-18512
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- A Family of Delayed Rectifier Kv1 cDNAs Showing Cell Type-Specific Expression in the Squid Stellate Ganglion/Giant Fiber Lobe Complex.
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J. Neurosci.
17, 5070-5079
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- Identification and Functional Characterization of a K+ Channel alpha -Subunit with Regulatory Properties Specific to Brain.
- A. Castellano, M. D. Chiara, B. Mellstrom, A. Molina, F. Monje, J. R. Naranjo, and J. Lopez-Barneo (1997)
J. Neurosci.
17, 4652-4661
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- Kvbeta 2 Inhibits the Kvbeta 1-mediated Inactivation of K+ Channels in Transfected Mammalian Cells.
- J. Xu and M. Li (1997)
J. Biol. Chem.
272, 11728-11735
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- Molecular Determinants for Assembly of G-protein-activated Inwardly Rectifying K+ Channels.
- R. Woodward, E. B. Stevens, and R. D. Murrell-Lagnado (1997)
J. Biol. Chem.
272, 10823-10830
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- Voltage-dependent gating characteristics of the K+ channel KAT1 depend on the N and C termini.
- I. Marten and T. Hoshi (1997)
PNAS
94, 3448-3453
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- Topology of the Shaker Potassium Channel Probed with Hydrophilic Epitope Insertions.
- T. M. Shih and A. L. Goldin (1997)
J. Cell Biol.
136, 1037-1045
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- GKAP, a Novel Synaptic Protein That Interacts with the Guanylate Kinase-like Domain of the PSD-95/SAP90 Family of Channel Clustering Molecules.
- E. Kim, S. Naisbitt, Y.-P. Hsueh, A. Rao, A. Rothschild, A. M. Craig, and M. Sheng (1997)
J. Cell Biol.
136, 669-678
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- Potassium Channel alpha and beta Subunits Assemble in the Endoplasmic Reticulum.
- N. Nagaya and D. M. Papazian (1997)
J. Biol. Chem.
272, 3022-3027
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- Membrane Insertion, Glycosylation, and Oligomerization of Inositol Trisphosphate Receptors in a Cell-free Translation System.
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J. Biol. Chem.
272, 1579-1588
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- The Human Delta 1261 Mutation of the HERG Potassium Channel Results in a Truncated Protein That Contains a Subunit Interaction Domain and Decreases the Channel Expression.
- X. Li, J. Xu, and M. Li (1997)
J. Biol. Chem.
272, 705-708
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- rZIP, a RING-leucine zipper protein that regulates cell fate determination during Dictyostelium development.
- P Balint-Kurti, G Ginsburg, O Rivero-Lezcano, and A. Kimmel (1997)
Development
124, 1203-1213
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- A Novel Subunit for Shal K+ Channels Radically Alters Activation and Inactivation.
- T. Jegla and L. Salkoff (1997)
J. Neurosci.
17, 32-44
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- Voltage-gated K+ Channels Contain Multiple Intersubunit Association Sites.
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J. Biol. Chem.
271, 18904-18911
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