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Science 28 August 1992:
Vol. 257. no. 5074, pp. 1225 - 1230
DOI: 10.1126/science.1519059

Articles

Science, Vol 257, Issue 5074, 1225-1230
Copyright © 1992 by American Association for the Advancement of Science


articles

Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel

M Li, YN Jan, and LY Jan

Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.

The functional heterogeneity of potassium channels in eukaryotic cells arises not only from the multiple potassium channel genes and splice variants but also from the combinatorial mixing of different potassium channel polypeptides to form heteromultimeric channels with distinct properties. One structural element that determines the compatibility of different potassium channel polypeptides in subunit assembly has now been localized to the hydrophilic amino-terminal domain. A Drosophila Shaker B (ShB) potassium channel truncated polypeptide that contains only the hydrophilic amino-terminal domain can form a homomultimer; the minimal requirement for the homophilic interaction has been localized to a fragment of 114 amino acids. Substitution of the amino-terminal domain of a distantly related mammalian potassium channel polypeptide (DRK1) with that of ShB permits the chimeric DRK1 polypeptide to coassemble with ShB.


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Targeted Expression of a Dominant-Negative Kv4.2 K+ Channel Subunit in the Mouse Heart.
A. D. Wickenden, P. Lee, R. Sah, Q. Huang, G. I. Fishman, and P. H. Backx (1999)
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M. L. Chalfant, J. S. Denton, A. L. Langloh, K. H. Karlson, J. Loffing, D. J. Benos, and B. A. Stanton (1999)
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Subunit-Specific Association of Protein Kinase C and the Receptor for Activated C Kinase with GABA Type A Receptors.
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D. J Benos and B. A Stanton (1999)
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F. Lehmann-Horn and K. Jurkat-Rott (1999)
Physiol Rev 79, 1317-1372
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A. Singh and S. Singh (1999)
J. Neurosci. 19, 6838-6843
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A Small Domain in the N Terminus of the Regulatory alpha -Subunit Kv2.3 Modulates Kv2.1 Potassium Channel Gating.
M. D. Chiara, F. Monje, A. Castellano, and J. Lopez-Barneo (1999)
J. Neurosci. 19, 6865-6873
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The Molecular Assembly of ATP-sensitive Potassium Channels. DETERMINANTS ON THE PORE FORMING SUBUNIT.
J. P. Giblin, J. L. Leaney, and A. Tinker (1999)
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Structure and function of cardiac potassium channels.
D. J Snyders (1999)
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R. Mathur, J. Zhou, T. Babila, and G. Koren (1999)
J. Biol. Chem. 274, 11487-11493
   Abstract »    Full Text »    PDF »
Protein Interactions with the Glucose Transporter Binding Protein That Provide a Link between GLUT1 and the Cytoskeleton.
R. C. Bunn, M. A. Jensen, and B. C. Reed (1999)
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Heteromultimeric Potassium Channels Formed by Members of the Kv2 Subfamily.
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Stoichiometry and arrangement of heteromeric olfactory cyclic nucleotide-gated ion channels.
M. S. Shapiro and W. N. Zagotta (1998)
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Subunit Folding and Assembly Steps Are Interspersed during Shaker Potassium Channel Biogenesis.
C. T. Schulteis, N. Nagaya, and D. M. Papazian (1998)
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Modulation of potassium channel gating by coexpression of Kv2.1 with regulatory Kv5.1 or Kv6.1 alpha -subunits.
J. W. Kramer, M. A. Post, A. M. Brown, and G. E. Kirsch (1998)
Am J Physiol Cell Physiol 274, C1501-C1510
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The transient outward current in mice lacking the potassium channel gene Kv1.4.
B. London, D. W Wang, J. A Hill, and P. B Bennett (1998)
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B. A. Wible, Q. Yang, Y. A. Kuryshev, Eric. A. Accili, and A. M. Brown (1998)
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C. P. Thomas, S. Auerbach, J. B. Stokes, and K. A. Volk (1998)
Am J Physiol Cell Physiol 274, C1312-C1323
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Long QT and ventricular arrhythmias in transgenic mice expressing the N terminus and first transmembrane segment of a voltage-gated potassium channel.
B. London, A. Jeron, J. Zhou, P. Buckett, X. Han, G. F. Mitchell, and G. Koren (1998)
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Number and Stoichiometry of Subunits in the Native Atrial G-protein-gated K+ Channel, IKACh.
S. Corey, G. Krapivinsky, L. Krapivinsky, and D. E. Clapham (1998)
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A State-independent Interaction between Ligand and a Conserved Arginine Residue in Cyclic Nucleotide-gated Channels Reveals a Functional Polarity of the Cyclic Nucleotide Binding Site.
G. R. Tibbs, D. T. Liu, B. G. Leypold, and S. A. Siegelbaum (1998)
J. Biol. Chem. 273, 4497-4505
   Abstract »    Full Text »    PDF »
Distinct functional stoichiometry of potassium channel beta  subunits.
J. Xu, W. Yu, J. M. Wright, R. W. Raab, and M. Li (1998)
PNAS 95, 1846-1851
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Suppression of Neuronal and Cardiac Transient Outward Currents by Viral Gene Transfer of Dominant-Negative Kv4.2 Constructs.
D. C. Johns, H. B. Nuss, and E. Marban (1997)
J. Biol. Chem. 272, 31598-31603
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Contribution of the NH2 terminus of Kv2.1 to channel activation.
J. M. Pascual, C.-C. Shieh, G. E. Kirsch, and A. M. Brown (1997)
Am J Physiol Cell Physiol 273, C1849-C1858
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Alternative Splicing in the Pore-Forming Region of shaker Potassium Channels.
M. Kim, D. J. Baro, C. C. Lanning, M. Doshi, J. Farnham, H. S. Moskowitz, J. H. Peck, B. M. Olivera, and R. M. Harris-Warrick (1997)
J. Neurosci. 17, 8213-8224
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Interactions between Subunits of the Human Epithelial Sodium Channel.
C. M. Adams, P. M. Snyder, and M. J. Welsh (1997)
J. Biol. Chem. 272, 27295-27300
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A Cellular Model for Long QT Syndrome. TRAPPING OF HETEROMULTIMERIC COMPLEXES CONSISTING OF TRUNCATED Kv1.1 POTASSIUM CHANNEL POLYPEPTIDES AND NATIVE Kv1.4 AND Kv1.5 CHANNELS IN THE ENDOPLASMIC RETICULUM.
E. Folco, R. Mathur, Y. Mori, P. Buckett, and G. Koren (1997)
J. Biol. Chem. 272, 26505-26510
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Interdomain Interactions Underlying Activation of Cyclic Nucleotide-Gated Channels.
M. D. Varnum and W. N. Zagotta (1997)
Science 278, 110-113
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M. Jiang, J. Tseng-Crank, and G.-N. Tseng (1997)
J. Biol. Chem. 272, 24109-24112
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D. J. Baro, R. M. Levini, M. T. Kim, A. R. Willms, C. C. Lanning, H. E. Rodriguez, and R. M. Harris-Warrick (1997)
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J. J. C. Rosenthal, T. I. Liu, and W. F. Gilly (1997)
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Identification and Functional Characterization of a K+ Channel alpha -Subunit with Regulatory Properties Specific to Brain.
A. Castellano, M. D. Chiara, B. Mellstrom, A. Molina, F. Monje, J. R. Naranjo, and J. Lopez-Barneo (1997)
J. Neurosci. 17, 4652-4661
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Kvbeta 2 Inhibits the Kvbeta 1-mediated Inactivation of K+ Channels in Transfected Mammalian Cells.
J. Xu and M. Li (1997)
J. Biol. Chem. 272, 11728-11735
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Molecular Determinants for Assembly of G-protein-activated Inwardly Rectifying K+ Channels.
R. Woodward, E. B. Stevens, and R. D. Murrell-Lagnado (1997)
J. Biol. Chem. 272, 10823-10830
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Voltage-dependent gating characteristics of the K+ channel KAT1 depend on the N and C termini.
I. Marten and T. Hoshi (1997)
PNAS 94, 3448-3453
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Topology of the Shaker Potassium Channel Probed with Hydrophilic Epitope Insertions.
T. M. Shih and A. L. Goldin (1997)
J. Cell Biol. 136, 1037-1045
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GKAP, a Novel Synaptic Protein That Interacts with the Guanylate Kinase-like Domain of the PSD-95/SAP90 Family of Channel Clustering Molecules.
E. Kim, S. Naisbitt, Y.-P. Hsueh, A. Rao, A. Rothschild, A. M. Craig, and M. Sheng (1997)
J. Cell Biol. 136, 669-678
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Potassium Channel alpha and beta Subunits Assemble in the Endoplasmic Reticulum.
N. Nagaya and D. M. Papazian (1997)
J. Biol. Chem. 272, 3022-3027
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Membrane Insertion, Glycosylation, and Oligomerization of Inositol Trisphosphate Receptors in a Cell-free Translation System.
S. K. Joseph, D. Boehning, S. Pierson, and C. V. Nicchitta (1997)
J. Biol. Chem. 272, 1579-1588
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The Human Delta 1261 Mutation of the HERG Potassium Channel Results in a Truncated Protein That Contains a Subunit Interaction Domain and Decreases the Channel Expression.
X. Li, J. Xu, and M. Li (1997)
J. Biol. Chem. 272, 705-708
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rZIP, a RING-leucine zipper protein that regulates cell fate determination during Dictyostelium development.
P Balint-Kurti, G Ginsburg, O Rivero-Lezcano, and A. Kimmel (1997)
Development 124, 1203-1213
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A Novel Subunit for Shal K+ Channels Radically Alters Activation and Inactivation.
T. Jegla and L. Salkoff (1997)
J. Neurosci. 17, 32-44
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Voltage-gated K+ Channels Contain Multiple Intersubunit Association Sites.
L. Tu, V. Santarelli, Z. Sheng, W. Skach, D. Pain, and C. Deutsch (1996)
J. Biol. Chem. 271, 18904-18911
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