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Science 14 August 1992:
Vol. 257. no. 5072, pp. 967 - 971
DOI: 10.1126/science.1354393

Articles

Science, Vol 257, Issue 5072, 967-971
Copyright © 1992 by American Association for the Advancement of Science


articles

Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction

P Liang and AB Pardee

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115.

Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under altered conditions. This report describes a method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction. The key element is to use a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer. The mRNA subpopulations defined by these primer pairs were amplified after reverse transcription and resolved on a DNA sequencing gel. When multiple primer sets were used, reproducible patterns of amplified complementary DNA fragments were obtained that showed strong dependence on sequence specificity of either primer.


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Rat endometrial Vdup1 expression: changes related to sensitization for the decidual cell reaction and hormonal control.
D. G Simmons and T. G Kennedy (2004)
Reproduction 127, 475-482
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Different Testicular Gene Expression Patterns in the First Spermatogenic Cycle of Postnatal and Vitamin A-Deficient Rat Testis.
K.-F. Lee, W. S.B. Yeung, J. F.C. Chow, C. K. Shum, and J. M. Luk (2004)
Biol Reprod 70, 1010-1017
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NGEP, a gene encoding a membrane protein detected only in prostate cancer and normal prostate.
T. K. Bera, S. Das, H. Maeda, R. Beers, C. D. Wolfgang, V. Kumar, Y. Hahn, B. Lee, and I. Pastan (2004)
PNAS 101, 3059-3064
   Abstract »    Full Text »    PDF »
Differential Expression of Desulfovibrio vulgaris Genes in Response to Cu(II) and Hg(II) Toxicity.
I. S. Chang, J. L. Groh, M. M. Ramsey, J. D. Ballard, and L. R. Krumholz (2004)
Appl. Envir. Microbiol. 70, 1847-1851
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Sexual Dimorphism of Rat Liver Gene Expression: Regulatory Role of Growth Hormone Revealed by Deoxyribonucleic Acid Microarray Analysis.
A. Ahluwalia, K. H. Clodfelter, and D. J. Waxman (2004)
Mol. Endocrinol. 18, 747-760
   Abstract »    Full Text »    PDF »
Activity-Dependent Expression of Acyl-Coenzyme A-Binding Protein in Retinal Muller Glial Cells Evoked by Optokinetic Stimulation.
N. H. Barmack, T. R. Bilderback, H. Liu, Z. Qian, and V. Yakhnitsa (2004)
J. Neurosci. 24, 1023-1033
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Differential Gene Expression in Auristatin PHE-Treated Cryptococcus neoformans.
T. Woyke, M. E. Berens, D. B. Hoelzinger, G. R. Pettit, G. Winkelmann, and R. K. Pettit (2004)
Antimicrob. Agents Chemother. 48, 561-567
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Mycobacterium bovis BCG Cell Wall-Specific Differentially Expressed Genes Identified by Differential Display and cDNA Subtraction in Human Macrophages.
N. A. Begum, K. Ishii, M. Kurita-Taniguchi, M. Tanabe, M. Kobayashi, Y. Moriwaki, M. Matsumoto, Y. Fukumori, I. Azuma, K. Toyoshima, et al. (2004)
Infect. Immun. 72, 937-948
   Abstract »    Full Text »    PDF »
Functional genomics approach to hypoxia signaling.
K. A. Seta and D. E. Millhorn (2004)
J Appl Physiol 96, 765-773
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