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Science 7 August 1992:
Vol. 257. no. 5071, pp. 800 - 803
DOI: 10.1126/science.1496400
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Articles

Science, Vol 257, Issue 5071, 800-803
Copyright © 1992 by American Association for the Advancement of Science

articles

Hypovirulence of chestnut blight fungus conferred by an infectious viral cDNA

GH Choi and DL Nuss

Department of Molecular Oncology and Virology, Roche Institute of Molecular Biology, Nutley, NJ 07110.

Strains of the chestnut blight fungus Cryphonectria parasitica that contain viral double-stranded RNAs often exhibit reduced virulence. Such hypovirulent strains act as biocontrol agents by virtue of their ability to convert virulent strains to hypovirulence after anastomosis. Transformation of virulent C. parasitica strains with a full-length complementary DNA copy of a hypovirulence-associated viral RNA conferred the complete hypovirulence phenotype. Cytoplasmic double-stranded RNA was resurrected from the chromosomally integrated complementary DNA copy and was able to convert compatible virulent strains to hypovirulence. These results establish viral double-stranded RNA as the casual agent of hypovirulence and demonstrate the feasibility of engineering hypovirulent fungal strains.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713.
L.-h. Guo, L. Sun, S. Chiba, H. Araki, and N. Suzuki (2009)
Nucleic Acids Res. 37, 3645-3659
   Abstract »    Full Text »    PDF »
Hypovirus-Responsive Transcription Factor Gene pro1 of the Chestnut Blight Fungus Cryphonectria parasitica Is Required for Female Fertility, Asexual Spore Development, and Stable Maintenance of Hypovirus Infection.
Q. Sun, G. H. Choi, and D. L. Nuss (2009)
Eukaryot. Cell 8, 262-270
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Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713.
L. Sun and N. Suzuki (2008)
RNA 14, 2557-2571
   Abstract »    Full Text »    PDF »
Hypovirus Papain-Like Protease p48 Is Required for Initiation but Not for Maintenance of Virus RNA Propagation in the Chestnut Blight Fungus Cryphonectria parasitica.
F. Deng and D. L. Nuss (2008)
J. Virol. 82, 6369-6378
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A Host Factor Involved in Hypovirus Symptom Expression in the Chestnut Blight Fungus, Cryphonectria parasitica.
M. I. Faruk, A. Eusebio-Cope, and N. Suzuki (2008)
J. Virol. 82, 740-754
   Abstract »    Full Text »    PDF »
Comparative Analysis of Alterations in Host Phenotype and Transcript Accumulation following Hypovirus and Mycoreovirus Infections of the Chestnut Blight Fungus Cryphonectria parasitica.
F. Deng, T. D. Allen, B. I. Hillman, and D. L. Nuss (2007)
Eukaryot. Cell 6, 1286-1298
   Abstract »    Full Text »    PDF »
Genome Sequence, Full-Length Infectious cDNA Clone, and Mapping of Viral Double-Stranded RNA Accumulation Determinant of Hypovirus CHV1-EP721.
H. Lin, X. Lan, H. Liao, T. B. Parsley, D. L. Nuss, and B. Chen (2007)
J. Virol. 81, 1813-1820
   Abstract »    Full Text »    PDF »
Synergism between a mycoreovirus and a hypovirus mediated by the papain-like protease p29 of the prototypic hypovirus CHV1-EP713.
L. Sun, D. L. Nuss, and N. Suzuki (2006)
J. Gen. Virol. 87, 3703-3714
   Abstract »    Full Text »    PDF »
Hypovirus Papain-Like Protease p29 Suppresses RNA Silencing in the Natural Fungal Host and in a Heterologous Plant System.
G. C. Segers, R. van Wezel, X. Zhang, Y. Hong, and D. L. Nuss (2006)
Eukaryot. Cell 5, 896-904
   Abstract »    Full Text »    PDF »
Microarray analysis of Cryphonectria parasitica G{alpha}- and G{beta}{gamma}-signalling pathways reveals extensive modulation by hypovirus infection.
A. L. Dawe, G. C. Segers, T. D. Allen, V. C. McMains, and D. L. Nuss (2004)
Microbiology 150, 4033-4043
   Abstract »    Full Text »    PDF »
Evidence for a Role of the Regulator of G-Protein Signaling Protein CPRGS-1 in G{alpha} Subunit CPG-1-Mediated Regulation of Fungal Virulence, Conidiation, and Hydrophobin Synthesis in the Chestnut Blight Fungus Cryphonectria parasitica.
G. C. Segers, J. C. Regier, and Donald. L. Nuss (2004)
Eukaryot. Cell 3, 1454-1463
   Abstract »    Full Text »    PDF »
Linkage between Mitochondrial Hypovirulence and Viral Hypovirulence in the Chestnut Blight Fungus Revealed by cDNA Microarray Analysis.
T. D. Allen and D. L. Nuss (2004)
Eukaryot. Cell 3, 1227-1232
   Abstract »    Full Text »    PDF »
Specific and Common Alterations in Host Gene Transcript Accumulation following Infection of the Chestnut Blight Fungus by Mild and Severe Hypoviruses.
T. D. Allen and D. L. Nuss (2004)
J. Virol. 78, 4145-4155
   Abstract »    Full Text »    PDF »
Hypovirus Papain-Like Protease p29 Functions in trans To Enhance Viral Double-Stranded RNA Accumulation and Vertical Transmission.
N. Suzuki, K. Maruyama, M. Moriyama, and D. L. Nuss (2003)
J. Virol. 77, 11697-11707
   Abstract »    Full Text »    PDF »
Differential Transfer and Dissemination of Hypovirus and Nuclear and Mitochondrial Genomes of a Hypovirus-Infected Cryphonectria parasitica Strain after Introduction into a Natural Population.
P. J. Hoegger, U. Heiniger, O. Holdenrieder, and D. Rigling (2003)
Appl. Envir. Microbiol. 69, 3767-3771
   Abstract »    Full Text »    PDF »
Transfection of Diaporthe perjuncta with Diaporthe RNA Virus.
N. Moleleki, S. W. van Heerden, M. J. Wingfield, B. D. Wingfield, and O. Preisig (2003)
Appl. Envir. Microbiol. 69, 3952-3956
   Abstract »    Full Text »    PDF »
Contribution of Protein p40 to Hypovirus-Mediated Modulation of Fungal Host Phenotype and Viral RNA Accumulation.
N. Suzuki and D. L. Nuss (2002)
J. Virol. 76, 7747-7759
   Abstract »    Full Text »    PDF »
Cryphonectria radicalis: rediscovery of a lost fungus.
P. J. Hoegger, D. Rigling, O. Holdenrieder, and U. Heiniger (2002)
Mycologia 94, 105-115
   Abstract »    Full Text »    PDF »
Using Chimeric Hypoviruses To Fine-Tune the Interaction between a Pathogenic Fungus and Its Plant Host.
B. Chen, L. M. Geletka, and D. L. Nuss (2000)
J. Virol. 74, 7562-7567
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Essential and Dispensable Virus-Encoded Replication Elements Revealed by Efforts To Develop Hypoviruses as Gene Expression Vectors.
N. Suzuki, L. M. Geletka, and D. L. Nuss (2000)
J. Virol. 74, 7568-7577
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Identification of bdm-1, a gene involved in G protein beta -subunit function and alpha -subunit accumulation.
S. Kasahara, P. Wang, and D. L. Nuss (2000)
PNAS 97, 412-417
   Abstract »    Full Text »    PDF »
Mapping of a Hypovirus p29 Protease Symptom Determinant Domain with Sequence Similarity to Potyvirus HC-Pro Protease.
N. Suzuki, B. Chen, and D. L. Nuss (1999)
J. Virol. 73, 9478-9484
   Abstract »    Full Text »    PDF »
Infectious cDNA Clone of Hypovirus CHV1-Euro7: a Comparative Virology Approach To Investigate Virus-Mediated Hypovirulence of the Chestnut Blight Fungus Cryphonectria parasitica.
B. Chen and D. L. Nuss (1999)
J. Virol. 73, 985-992
   Abstract »    Full Text »
Distinct roles for two G protein alpha  subunits in fungal virulence, morphology, and reproduction revealed by targeted gene disruption.
S. Gao and D. L. Nuss (1996)
PNAS 93, 14122-14127
   Abstract »    Full Text »    PDF »
Attenuation of fungal virulence by synthetic infectious hypovirus transcripts.
B Chen, G. Choi, and D. Nuss (1994)
Science 264, 1762-1764
   Abstract »    PDF »


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