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Science 31 July 1992:
Vol. 257. no. 5070, pp. 657 - 661
DOI: 10.1126/science.1353905
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Articles

Science, Vol 257, Issue 5070, 657-661
Copyright © 1992 by American Association for the Advancement of Science

articles

Calcium-dependent transmitter secretion reconstituted in Xenopus oocytes: requirement for synaptophysin

J Alder, B Lu, F Valtorta, P Greengard, and MM Poo

Department of Biological Sciences, Columbia University, New York, NY 10027.

Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA). Co-injection of total mRNA with antisense oligonucleotides to synaptophysin message decreased the expression of synaptophysin in the oocyte and reduced the calcium-dependent secretion. A similar effect on secretion was observed for oocytes injected with total mRNA together with an antibody to rat synaptophysin. These results indicate that synaptophysin is necessary for transmitter secretion and that the oocyte expression system may be useful for dissecting the molecular events associated with the secretory process.


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