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Science 10 July 1992:
Vol. 257. no. 5067, pp. 245 - 248
DOI: 10.1126/science.1321495
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Articles

Science, Vol 257, Issue 5067, 245-248
Copyright © 1992 by American Association for the Advancement of Science

articles

Phosphorylation-independent modulation of L-type calcium channels by magnesium-nucleotide complexes

B O'Rourke, PH Backx, and E Marban

Department of Medicine, Johns Hopkins University, Baltimore, MD 21205.

Free magnesium ions and magnesium-nucleotide complexes can exert opposite effects on many fundamental cellular processes. Although increases in the intracellular concentration of magnesium ions inhibit the L-type calcium current in heart cells, magnesium-adenosine triphosphate complexes (MgATP) would be expected to increase the current by promoting channel phosphorylation. Rapid increases in the intracellular concentration of MgATP induced by flash photolysis of caged magnesium or caged ATP resulted in enhanced calcium current. The increase in calcium current was not prevented by blocking phosphorylation, revealing a previously unrecognized direct regulatory action of the magnesium-nucleotide complex.


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