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Science 3 July 1992:
Vol. 257. no. 5066, pp. 94 - 96
DOI: 10.1126/science.1621101
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Articles

Science, Vol 257, Issue 5066, 94-96
Copyright © 1992 by American Association for the Advancement of Science

articles

No requirement for p56lck in the antigen-stimulated clonal deletion of thymocytes

K Nakayama and DY Loh

Howard Hughes Medical Institute, Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110.

Activation of protein-tyrosine kinases (PTKs) is required for signal transduction during T cell activation, although the pathway used during thymic selection is unknown. An in vitro system was established in which T cell receptor transgenic thymocytes underwent clonal deletion in response to peptide antigen. The effects of two PTK-specific inhibitors, herbimycin A and genistein, on the clonal deletion of immature thymocytes and the activation of mature thymocytes were examined. Clonal deletion occurred while T cell activation was inhibited and when no p56lck activity was evident. Thus, p56lck is not required for the antigen-stimulated step of clonal deletion of immature thymocytes, and negative selection proceeds via a distinct pathway.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rapid deletion of rearranged T cell antigen receptor (TCR) Valpha -Jalpha segment by secondary rearrangement in the thymus: Role of continuous rearrangement of TCR alpha  chain gene and positive selection in the T cell repertoire formation.
F. Wang, C.-Y. Huang, and O. Kanagawa (1998)
PNAS 95, 11834-11839
   Abstract »    Full Text »    PDF »
Viral Superantigen-Induced Negative Selection of TCR Transgenic CD4+ CD8+ Thymocytes Depends on Activation, but not Proliferation.
I. Ferrero, F. Anjuere, I. Azcoitia, T. Renno, H. R. MacDonald, and C. Ardavin (1998)
Blood 91, 4248-4254
   Abstract »    Full Text »    PDF »
Requirement for CD8 beta chain in positive selection of CD8-lineage T cells.
K Nakayama, K Nakayama, I Negishi, K Kuida, M. Louie, O Kanagawa, H Nakauchi, and D. Loh (1994)
Science 263, 1131-1133
   Abstract »    PDF »
Thymic selection of cytotoxic T cells independent of CD8 alpha-Lck association.
I. Chan, A Limmer, M. Louie, E. Bullock, W. Fung-Leung, T. Mak, and D. Loh (1993)
Science 261, 1581-1584
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