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Science 3 July 1992:
Vol. 257. no. 5066, pp. 76 - 80
DOI: 10.1126/science.1621097
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Articles

Science, Vol 257, Issue 5066, 76-80
Copyright © 1992 by American Association for the Advancement of Science

articles

Conformation of the TAR RNA-arginine complex by NMR spectroscopy

JD Puglisi, R Tan, BJ Calnan, AD Frankel, and Williamson JR

Department of Chemistry, Massachusetts Institute of Technology, Cambridge 02139.

The messenger RNAs of human immunodeficiency virus-1 (HIV-1) have an RNA hairpin structure, TAR, at their 5' ends that contains a six-nucleotide loop and a three-nucleotide bulge. The conformations of TAR RNA and of TAR with an arginine analog specifically bound at the binding site for the viral protein, Tat, were characterized by nuclear magnetic resonance (NMR) spectroscopy. Upon arginine binding, the bulge changes conformation, and essential nucleotides for binding, U23 and A27.U38, form a base-triple interaction that stabilizes arginine hydrogen bonding to G26 and phosphates. Specificity in the arginine-TAR interaction appears to be derived largely from the structure of the RNA.


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