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Science 29 May 1992:
Vol. 256. no. 5061, pp. 1333 - 1335
DOI: 10.1126/science.1598579
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Articles

Science, Vol 256, Issue 5061, 1333-1335
Copyright © 1992 by American Association for the Advancement of Science

articles

Nondissociation of GAL4 and GAL80 in vivo after galactose induction

KK Leuther and SA Johnston

Department of Medicine, University of Texas-Southwestern Medical Center, Dallas 75235.

Transcription of galactose-inducible genes in yeast is regulated by interaction between the activator protein GAL4 and the negative regulatory protein GAL80. It has been suggested that GAL80 binds to and represses GAL4 under uninduced conditions and dissociates from GAL4 on induction. However, the possibility that GAL80 remains associated with GAL4 after induction has not been ruled out. Experiments to discriminate between these two models were performed and revealed that GAL80 stays bound after induction.


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