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Science 29 May 1992:
Vol. 256. no. 5061, pp. 1324 - 1327
DOI: 10.1126/science.256.5061.1324
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Articles

Conjugative Transfer by the Virulence System of Agrobacterium tumefaciens

Alice Beijersbergen 1, Amke Den Dulk-Ras 1, Rob A. Schilperoort 1, and Paul J. J. Hooykaas 1

1 Institute of Molecular Plant Sciences, Clusius Laboratory, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

Agrobacterium tumefaciens transfers part of its Ti plasmid, the transferred DNA (T-DNA), to plant cells during tumor induction. Expression of this T-DNA in plant cells results in their transformation into tumor cells. There are similarities between the process of T-DNA transfer to plants and the process of bacterial conjugation. Here, the T-DNA transfer machinery mediated conjugation between bacteria. Thus, products of the Vir region of the Ti plasmid of Agrobacterium tumefaciens, normally involved in transfer of DNA from bacteria to plants, can direct the conjugative transfer of an IncQ plasmid between agrobacteria.

Submitted on November 1, 1991
Accepted on March 31, 1992


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Science. ISSN 0036-8075 (print), 1095-9203 (online)