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Science 22 May 1992:
Vol. 256. no. 5060, pp. 1215 - 1217
DOI: 10.1126/science.256.5060.1215
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Articles

G Protein Activation of a Hormone-Stimulated Phosphatase in Human Tumor Cells

Ming Gui Pan 1, Tullio Florio 2, and Philip J. S. Stork 1

1 The Vollum Institute for Advanced Biomedical Research and Department of Cell Biology and Anatomy, Oregon Health Sciences University, L474, 3181 SW Sam Jackson Park Road, Portland, OR 97201
2 The Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, L474, 3181 SW Sam Jackson Park Road, Portland, OR 97201

The growth-inhibiting peptide hormone somatostatin stimulates phosphotyrosine phosphatase activity in the human pancreatic cell line MIA PaCa-2. This hormonal activation was mediated by a pertussis toxin-sensitive guanosine 5'-triphosphate-binding protein (G protein) in the membranes of these cells. Activation of this G protein by somatostatin stimulated the dephosphorylation of exogenous epidermal growth factor receptor prepared from A-431 cells in vitro. This pathway may mediate the antineoplastic action of somatostatin in these cells and in human tumors and could represent a general mechanism of G protein coupling that is utilized by normal cells in the hormonal control of cell growth.


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