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Science 15 May 1992:
Vol. 256. no. 5059, pp. 1014 - 1018
DOI: 10.1126/science.1589769

Articles

Science, Vol 256, Issue 5059, 1014-1018
Copyright © 1992 by American Association for the Advancement of Science


articles

Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos

MA Blanar and WJ Rutter

Hormone Research Institute, University of California, San Francisco 94143.

A facile method for isolating genes that encode interacting proteins has been developed with a polypeptide probe that contains an amino-terminal extension with recognition sites for a monoclonal antibody, a specific endopeptidase, and a site-specific protein kinase. This probe, containing the basic region-leucine zipper dimerization motif of c-Fos, was used to screen a complementary DNA library. A complementary DNA that encoded a member of the basic-helix-loop-helix-zipper (bHLH-Zip) family of proteins was isolated. The complementary DNA-encoded polypeptide FIP (Fos interacting protein) bound to oligonucleotide probes that contained DNA binding motifs for other HLH proteins. When cotransfected with c-Fos, FIP stimulated transcription of an AP-1-responsive promoter.


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Conservation and Diversity of Eukaryotic Translation Initiation Factor eIF3.
K. Asano, T. G. Kinzy, W. C. Merrick, and J. W.B. Hershey (1997)
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Development 124, 1203-1213
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Transcription of the Transforming Growth Factor-beta 2 Gene Is Dependent on an E-box Located between an Essential cAMP Response Element/Activating Transcription Factor Motif and the TATA Box of the Gene.
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Drosophila alpha -Catenin and E-cadherin Bind to Distinct Regions of Drosophila Armadillo.
L.-M. Pai, C. Kirkpatrick, J. Blanton, H. Oda, M. Takeichi, and M. Peifer (1996)
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BID: a novel BH3 domain-only death agonist..
K Wang, X M Yin, D T Chao, C L Milliman, and S J Korsmeyer (1996)
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The Kinase Insert Domain of Interferon-induced Protein Kinase PKR Is Required for Activity but Not for Interaction with the Pseudosubstrate K3L.
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DNA Binding by the Heterodimeric Ah Receptor.
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Immunochemical Characterization and Transacting Properties of Upstream Stimulatory Factor Isoforms.
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E2F-4, a new member of the E2F gene family, has oncogenic activity and associates with p107 in vivo..
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