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Science 8 May 1992:
Vol. 256. no. 5058, pp. 836 - 839
DOI: 10.1126/science.1375394
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Articles

Science, Vol 256, Issue 5058, 836-839
Copyright © 1992 by American Association for the Advancement of Science

articles

Initiation of deregulated growth of multipotent progenitor cells by bcr-abl in vitro

ML Gishizky and ON Witte

Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024.

Expression of the bcr-abl oncogene in multipotent progenitor cells (MPPCs) is implicated as a key event in the development of chronic myelogenous leukemia. Bone marrow enriched for MPPCs was infected with a retrovirus that carried bcr-abl. The mixed-lineage colonies that resulted were responsive to growth factors and could differentiate. These cells later became growth factor-independent but, when injected into severe combined immunodeficient mice, were not leukemogenic. Thus, the presence of bcr-abl alone does not cause growth factor independence, although it initiates a stepwise process. This system may prove useful in the study of other oncogenes that cause leukemia.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)