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Science 17 April 1992:
Vol. 256. no. 5055, pp. 379 - 382
DOI: 10.1126/science.256.5055.379
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Articles

Reciprocal Regulation of Adipogenesis by Myc and C/EBPagr

Svend O. Freytag 1 and Tim J. Geddes 2

1 Molecular Biology Research Program, Henry Ford Hospital, Detroit, Ml 48202, and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Ml 48109
2 Molecular Biology Research Program, Henry Ford Hospital, Detroit, Ml 48202

3T3-L1 adipoblasts that express large amounts of c-Myc cannot terminally differentiate, raising the possibility that Myc inhibits the expression of genes that promote adipogenesis. The CCAAT/enhancer binding protein (C/EBPagr) is induced during 3T3-L1 adipogenesis when cells commit to the differentiation pathway. Transfection of 3T3-L1 adipoblasts with the gene that encodes C/EBPagr caused overt expression of the adipocyte morphology. Expression of Myc prohibited the normal induction of C/EBPagr and prevented adipogenesis. Enforced expression of C/EBPagr overcame the Myc-induced block to differentiation. These results provide a molecular basis for the regulation of adipogenesis and implicate Myc and C/EBPagr as pivotal controlling elements.


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Functional Antagonism between Msx2 and CCAAT/Enhancer-binding Protein alpha in Regulating the Mouse Amelogenin Gene Expression Is Mediated by Protein-Protein Interaction.
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