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Science 17 April 1992:
Vol. 256. no. 5055, pp. 377 - 379
DOI: 10.1126/science.256.5055.377
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Articles

Nucleoid Condensation in Escherichia coli That Express a Chlamydial Histone Homolog

Clifton E. Barry III 1, Stanley F. Hayes 2, and Ted Hackstadt 1

1 Laboratory of Intracellular Parasites, Host-Parasite Interaction Unit, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840
2 Laboratory of Vectors and Pathogens, Structural Pathobiology Section, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, Hamilton, MT 59840

Chlamydial cell types are adapted for either extracellular survival or intracellular growth. In the transcriptionally inert elementary bodies, the chromosome is densely compacted; in metabolically active reticulate bodies, the chromatin is loosely organized. Condensation of the chlamydial nucleoid occurs concomitant with expression of proteins homologous to eukaryotic histone H1. When the Chlamydia trachomatis 18-kilodalton histone homolog Hc1 is expressed in Escherichia coli, a condensed nucleoid structure similar to that of chlamydiae is observed with both light and electron microscopy. These results support a role for Hc1 in condensation of the chlamydial nucleoid.


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