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Science 10 April 1992:
Vol. 256. no. 5054, pp. 237 - 240
DOI: 10.1126/science.1566072
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Articles

Science, Vol 256, Issue 5054, 237-240
Copyright © 1992 by American Association for the Advancement of Science

articles

Positive control of pre-mRNA splicing in vitro

M Tian and T Maniatis

Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, MA 02138.

Positive control of the sex-specific alternative splicing of doublesex (dsx) precursor messenger RNA (pre-mRNA) in Drosophila melanogaster involves the activation of a female-specific 3' splice site by the products of the transformer (tra) and transformer-2 (tra-2) genes. The mechanisms of this process were investigated in an in vitro system in which the female-specific 3' splice site could be activated by recombinant Tra or Tra-2 (or both). An exon sequence essential for regulation in vivo was shown to be both necessary and sufficient for activation in vitro. Nuclear proteins in addition to Tra and Tra-2 were found to bind specifically to this exon sequence. Therefore, Tra and Tra-2 may act by promoting the assembly of a multiprotein complex on the exon sequence. This complex may facilitate recognition of the adjacent 3' splice site by the splicing machinery.


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The SRm160/300 splicing coactivator is required for exon-enhancer function.
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In vivo analysis of the functional domains of the Drosophila splicing regulator RBP1.
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The Mammalian Homolog of Suppressor-of-white-apricot Regulates Alternative mRNA Splicing of CD45 Exon 4and Fibronectin IIICS.
M. Sarkissian, A. Winne, and R. Lafyatis (1996)
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Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability..
J Wang, Y Takagaki, and J L Manley (1996)
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Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila doublesex splicing enhancer..
K W Lynch and T Maniatis (1996)
Genes & Dev. 10, 2089-2101
   Abstract »    PDF »
SR proteins and splicing control..
J L Manley and R Tacke (1996)
Genes & Dev. 10, 1569-1579
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The splicing factor U2AF35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing..
P Zuo and T Maniatis (1996)
Genes & Dev. 10, 1356-1368
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The mec-8 gene of C. elegans encodes a protein with two RNA recognition motifs and regulates alternative splicing of unc-52 transcripts.
E. Lundquist, R. Herman, T. Rogalski, G. Mullen, D. Moerman, and J. Shaw (1996)
Development 122, 1601-1610
   Abstract »    PDF »
The generally expressed hnRNP F is involved in a neural-specific pre-mRNA splicing event..
H Min, R C Chan, and D L Black (1995)
Genes & Dev. 9, 2659-2671
   Abstract »    PDF »
Soma-specific expression and cloning of PSI, a negative regulator of P element pre-mRNA splicing..
C W Siebel, A Admon, and D C Rio (1995)
Genes & Dev. 9, 269-283
   Abstract »    PDF »
Synergistic interactions between two distinct elements of a regulated splicing enhancer..
K W Lynch and T Maniatis (1995)
Genes & Dev. 9, 284-293
   Abstract »    PDF »
A splicing enhancer exhibits both constitutive and regulated activities..
M Tian and T Maniatis (1994)
Genes & Dev. 8, 1703-1712
   Abstract »    PDF »
Regulation of alternative pre-mRNA splicing by a novel repeated hexanucleotide element..
G S Huh and R O Hynes (1994)
Genes & Dev. 8, 1561-1574
   Abstract »    PDF »
Regulation of splicing at an intermediate step in the formation of the spliceosome..
J Vilardell and J R Warner (1994)
Genes & Dev. 8, 211-220
   Abstract »    PDF »
A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding..
A Lavigueur, H La Branche, A R Kornblihtt, and B Chabot (1993)
Genes & Dev. 7, 2405-2417
   Abstract »    PDF »
General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer..
Q Sun, A Mayeda, R K Hampson, A R Krainer, and F M Rottman (1993)
Genes & Dev. 7, 2598-2608
   Abstract »    PDF »
Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA..
S W Ruby, T H Chang, and J Abelson (1993)
Genes & Dev. 7, 1909-1925
   Abstract »    PDF »
A functional association between the 5' and 3' splice site is established in the earliest prespliceosome complex (E) in mammals..
S Michaud and R Reed (1993)
Genes & Dev. 7, 1008-1020
   Abstract »    PDF »
The role of exon sequences in splice site selection..
A Watakabe, K Tanaka, and Y Shimura (1993)
Genes & Dev. 7, 407-418
   Abstract »    PDF »
U1 snRNP targets an essential splicing factor, U2AF65, to the 3' splice site by a network of interactions spanning the exon..
B E Hoffman and P J Grabowski (1992)
Genes & Dev. 6, 2554-2568
   Abstract »    PDF »
The mechanism of somatic inhibition of Drosophila P-element pre-mRNA splicing: multiprotein complexes at an exon pseudo-5' splice site control U1 snRNP binding..
C W Siebel, L D Fresco, and D C Rio (1992)
Genes & Dev. 6, 1386-1401
   Abstract »    PDF »
Strong RNA Splicing Enhancers Identified by a Modified Method of Cycled Selection Interact with SR Protein.
H. Tian and R. Kole (2001)
J. Biol. Chem. 276, 33833-33839
   Abstract »    Full Text »    PDF »
Binding of PurH to a Muscle-specific Splicing Enhancer Functionally Correlates with Exon Inclusion in Vivo.
K. J. Ryan, N. Charlet-B., and T. A. Cooper (2000)
J. Biol. Chem. 275, 20618-20626
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)