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Science 10 April 1992:
Vol. 256. no. 5054, pp. 219 - 221
DOI: 10.1126/science.1566068
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Articles

Science, Vol 256, Issue 5054, 219-221
Copyright © 1992 by American Association for the Advancement of Science

articles

Centriole duplication in lysates of Spisula solidissima oocytes

RE Palazzo, E Vaisberg, RW Cole, and CL Rieder

Marine Biological Laboratory, Woods Hole, MA 02543.

A cell-free system has been developed that executes centriole duplication. Surf clam (Spisula solidissima) oocytes, arrested at late prophase of meiosis I, do not contain centrioles, centrosomes, or asters. Serial section high-voltage electron microscopy (HVEM) of asters and spindles isolated from potassium chloride-activated oocytes indicates that within 4 minutes oocytes assemble a single centriole that is duplicated by 15 minutes when assembly of the first meiotic spindle is complete. A mixture of lysates from unactivated oocytes and potassium chloride-activated oocytes induces centriole formation and duplication. Astral microtubule content in these lysate mixtures increases with time.


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Centrosomal protein centrin is not detectable during early pre-implantation development but reappears during late blastocyst stage in porcine embryos..
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Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter.
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The de novo centriole assembly pathway in HeLa cells: cell cycle progression and centriole assembly/maturation.
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The arithmetic of centrosome biogenesis.
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De novo formation of centrosomes in vertebrate cells arrested during S phase.
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Reconstitution of microtubule nucleation potential in centrosomes isolated from Spisula solidissima oocytes.
B. Schnackenberg, D. Hull, R. Balczon, and R. Palazzo (2000)
J. Cell Sci. 113, 943-953
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Cyclin-dependent kinase control of centrosome duplication.
K. R. Lacey, P. K. Jackson, and T. Stearns (1999)
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Requirement of Cdk2-Cyclin E Activity for Repeated Centrosome Reproduction in Xenopus Egg Extracts.
E. H. Hinchcliffe, C. Li, E. A. Thompson, J. L. Maller, and G. Sluder (1999)
Science 283, 851-854
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Dynein is required for spindle assembly in cytoplasmic extracts of Spisula solidissima oocytes.
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The disassembly and reassembly of functional centrosomes in vitro.
B. J. Schnackenberg, A. Khodjakov, C. L. Rieder, and R. E. Palazzo (1998)
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Two proteins isolated from sea urchin sperm flagella: structural components common to the stable microtubules of axonemes and centrioles.
E. Hinchcliffe and R. Linck (1998)
J. Cell Sci. 111, 585-595
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Assembly and positioning of microtubule asters in microfabricated chambers.
T. E. Holy, M. Dogterom, B. Yurke, and S. Leibler (1997)
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Centrosomes Isolated from Spisula solidissima Oocytes Contain Rings and an Unusual Stoichiometric Ratio of alpha /beta Tubulin.
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Cell Cycle-coupled Relocation of Types I and II Topoisomerases and Modulation of Catalytic Enzyme Activities.
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Meiotic spindle organization in fertilized Drosophila oocyte: presence of centrosomal components in the meiotic apparatus.
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