Related Content
Search Google Scholar for:
|
|
Science 10 April 1992:
Vol. 256. no. 5054, pp. 198 - 203
DOI: 10.1126/science.1533056
|
|
Articles
Science, Vol 256, Issue 5054, 198-203
Copyright © 1992 by American Association for the Advancement of Science
Lambda Int protein bridges between higher order complexes at two distant chromosomal loci attL and attR
S Kim
and
A Landy
Division of Biology and Medicine, Brown University, Providence, RI 02912.
The excisive recombination reaction of bacteriophage lambda involves a specific and efficient juxtaposition of two distant higher order protein-DNA complexes on the chromosome of Escherichia coli. These complexes, which mediate synapsis and strand exchange, consist of two DNA sequences, attL and attR, the bivalent DNA binding protein Int, and the sequence-specific DNA bending proteins, IHF, Xis, and Fis. The protein-protein and protein-DNA interactions within, and between, these complexes were studied by various biochemical techniques and the patterns of synergism among pairs of mutants with marginally impaired recombination function were analyzed. The DNA bending proteins facilitated long-range tethering of high- and low-affinity DNA sites by the bivalent Int protein, and a specific map is proposed for the resulting Int bridges. These structural motifs provide a basis for postulating the mechanism of site-specific recombination and may also be relevant to other pathways in which two distant chromosomal sites become associated.
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
- The antiparallel loops in gal DNA.
- G. Lia, S. Semsey, D. E. A. Lewis, S. Adhya, D. Bensimon, D. Dunlap, and L. Finzi (2008)
Nucleic Acids Res.
36, 4204-4210
| Abstract »
| Full Text »
| PDF »
- A Biotin Interference Assay Highlights Two Different Asymmetric Interaction Profiles for {lambda} Integrase Arm-type Binding Sites in Integrative Versus Excisive Recombination.
- D. Hazelbaker, M. A. Azaro, and A. Landy (2008)
J. Biol. Chem.
283, 12402-12414
| Abstract »
| Full Text »
| PDF »
- Comparative analysis of FimB and FimE recombinase activity.
- N. Holden, I. C. Blomfield, B.-E. Uhlin, M. Totsika, D. H. Kulasekara, and D. L. Gally (2007)
Microbiology
153, 4138-4149
| Abstract »
| Full Text »
| PDF »
- IntDOT Interactions with Core- and Arm-Type Sites of the Conjugative Transposon CTnDOT.
- J. M. DiChiara, A. N. Mattis, and J. F. Gardner (2007)
J. Bacteriol.
189, 2692-2701
| Abstract »
| Full Text »
| PDF »
- Common and Variable Contributions of Fis Residues to High-Affinity Binding at Different DNA Sequences.
- L. S. Feldman-Cohen, Y. Shao, D. Meinhold, C. Miller, W. Colon, and R. Osuna (2006)
J. Bacteriol.
188, 2081-2095
| Abstract »
| Full Text »
| PDF »
- Inaugural Article: Architecture of recombination intermediates visualized by in-gel FRET of {lambda} integrase-Holliday junction-arm DNA complexes.
- M. Radman-Livaja, T. Biswas, D. Mierke, and A. Landy (2005)
PNAS
102, 3913-3920
| Abstract »
| Full Text »
| PDF »
- Mutations at Residues 282, 286, and 293 of Phage {lambda} Integrase Exert Pathway-Specific Effects on Synapsis and Catalysis in Recombination.
- T. M. Bankhead, B. J. Etzel, F. Wolven, S. Bordenave, J. L. Boldt, T. A. Larsen, and A. M. Segall (2003)
J. Bacteriol.
185, 2653-2666
| Abstract »
| Full Text »
| PDF »
- Conservation of structure and function among tyrosine recombinases: homology-based modeling of the lambda integrase core-binding domain.
- B. M. Swalla, R. I. Gumport, and J. F. Gardner (2003)
Nucleic Acids Res.
31, 805-818
| Abstract »
| Full Text »
| PDF »
- Interactions between Integrase and Excisionase in the Phage Lambda Excisive Nucleoprotein Complex.
- E. H. Cho, R. I. Gumport, and J. F. Gardner (2002)
J. Bacteriol.
184, 5200-5203
| Abstract »
| Full Text »
| PDF »
- {lambda} Integrase Complementation at the Level of DNA Binding and Complex Formation.
- S. E. Nunes-Duby, M. Radman-Livaja, R. G. Kuimelis, R. V. Pearline, L. W. McLaughlin, and A. Landy (2002)
J. Bacteriol.
184, 1385-1394
| Abstract »
| Full Text »
| PDF »
- NF-{{kappa}}B p50-Dependent In Vivo Footprints at Ig S{{gamma}}3 DNA Are Correlated with {micro}{->}{{gamma}}3 Switch Recombination.
- R. A. Wuerffel, L. Ma, and A. L. Kenter (2001)
J. Immunol.
166, 4552-4559
| Abstract »
| Full Text »
| PDF »
- Transcriptional activation by LR1 at the E{micro} enhancer and switch region sites.
- L. A. Hanakahi and N. Maizels (2000)
Nucleic Acids Res.
28, 2651-2657
| Abstract »
| Full Text »
| PDF »
- Two Widely Spaced Initiator Binding Sites Create an HMG1-Dependent Parvovirus Rolling-Hairpin Replication Origin.
- S. F. Cotmore, J. Christensen, and P. Tattersall (2000)
J. Virol.
74, 1332-1341
| Abstract »
| Full Text »
- Interactions of the Integrase Protein of the Conjugative Transposon Tn916 with Its Specific DNA Binding Sites.
- Y. Jia and G. Churchward (1999)
J. Bacteriol.
181, 6114-6123
| Abstract »
| Full Text »
- In Vitro Selection of Integration Host Factor Binding Sites.
- S. D. Goodman, N. J. Velten, Q. Gao, S. Robinson, and A. M. Segall (1999)
J. Bacteriol.
181, 3246-3255
| Abstract »
| Full Text »
- Analysis of Higher Order Intermediates and Synapsis in the Bent-L Pathway of Bacteriophage lambda Site-specific Recombination.
- A. M. Segall (1998)
J. Biol. Chem.
273, 24258-24265
| Abstract »
| Full Text »
| PDF »
- A novel host factor for integration of mycobacteriophage L5.
- M. L. Pedulla, M. H. Lee, D. C. Lever, and G. F. Hatfull (1996)
PNAS
93, 15411-15416
| Abstract »
| Full Text »
| PDF »
- Architectural transcription factors.
- A. Wolffe (1994)
Science
264, 1100-1101
| PDF »
- Characterization of a Mutation of Bacteriophage lambda Integrase. PUTATIVE ROLE IN CORE BINDING AND STRAND EXCHANGE FOR A CONSERVED RESIDUE.
- T. Bankhead and A. M. Segall (2000)
J. Biol. Chem.
275, 36949-36956
| Abstract »
| Full Text »
| PDF »
- Assembly and activation of site-specific recombination complexes.
- C. E. A. Pena, J. M. Kahlenberg, and G. F. Hatfull (2000)
PNAS
97, 7760-7765
| Abstract »
| Full Text »
| PDF »
|
|
