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Science 27 March 1992:
Vol. 255. no. 5052, pp. 1712 - 1715
DOI: 10.1126/science.1553560
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Articles

Science, Vol 255, Issue 5052, 1712-1715
Copyright © 1992 by American Association for the Advancement of Science

articles

The size of gating charge in wild-type and mutant Shaker potassium channels

NE Schoppa, K McCormack, MA Tanouye, and FJ Sigworth

Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510.

The high sensitivity of voltage-gated ion channels to changes in membrane potential implies that the process of channel opening is accompanied by large charge movements. Previous estimates of the total charge displacement, q, have been deduced from the voltage dependence of channel activation and have ranged from 4 to 8 elementary charges (e0). A more direct measurement of q in Drosophila melanogaster Shaker 29-4 potassium channels yields a q value of 12.3 e0. A similar q value is obtained from mutated Shaker channels having reduced voltage sensitivity. These results can be explained by a model for channel activation in which the equilibria of voltage-dependent steps are altered in the mutant channels.


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Voltage-controlled gating in a large conductance Ca2+-sensitive K+channel (hslo).
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Intrinsic Voltage Dependence and Ca2+ Regulation of mslo Large Conductance Ca-activated K+ Channels.
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{blacksquare} REVIEW : Ionic Channels with Weak Voltage-dependence.
M. A. Rizzo (1997)
Neuroscientist 3, 102-111
   Abstract »    PDF »
Role of the S3-S4 Linker in Shaker Potassium Channel Activation.
R. Mathur, J. Zheng, Y. Yan, and F. J. Sigworth (1997)
J. Gen. Physiol. 109, 191-199
   Abstract »    Full Text »    PDF »
Total Charge Movement per Channel: The Relation between Gating Charge Displacement and the Voltage Sensitivity of Activation.
D. Sigg and F. Bezanilla (1997)
J. Gen. Physiol. 109, 27-39
   Abstract »    Full Text »    PDF »
Gating current noise produced by elementary transitions in Shaker potassium channels.
D Sigg, E Stefani, and F Bezanilla (1994)
Science 264, 578-582
   Abstract »    PDF »
Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.
D. Schachtman, J. Schroeder, W. Lucas, J. Anderson, and R. Gaber (1992)
Science 258, 1654-1658
   Abstract »    PDF »
Inaugural Article: Periodic perturbations in Shaker K+ channel gating kinetics by deletions in the S3-S4 linker.
C. Gonzalez, E. Rosenman, F. Bezanilla, O. Alvarez, and R. Latorre (2001)
PNAS 98, 9617-9623
   Abstract »    Full Text »    PDF »
Mutations throughout the S6 region of the hKv1.5 channel alter the stability of the activation gate.
T. C. Rich, S. W. Yeola, M. M. Tamkun, and D. J. Snyders (2002)
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   Abstract »    Full Text »    PDF »


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