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Science 27 March 1992:
Vol. 255. no. 5052, pp. 1712 - 1715
DOI: 10.1126/science.1553560
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Articles

Science, Vol 255, Issue 5052, 1712-1715
Copyright © 1992 by American Association for the Advancement of Science

articles

The size of gating charge in wild-type and mutant Shaker potassium channels

NE Schoppa, K McCormack, MA Tanouye, and FJ Sigworth

Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510.

The high sensitivity of voltage-gated ion channels to changes in membrane potential implies that the process of channel opening is accompanied by large charge movements. Previous estimates of the total charge displacement, q, have been deduced from the voltage dependence of channel activation and have ranged from 4 to 8 elementary charges (e0). A more direct measurement of q in Drosophila melanogaster Shaker 29-4 potassium channels yields a q value of 12.3 e0. A similar q value is obtained from mutated Shaker channels having reduced voltage sensitivity. These results can be explained by a model for channel activation in which the equilibria of voltage-dependent steps are altered in the mutant channels.


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