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Science 20 March 1992: Vol. 255. no. 5051, pp. 1573 - 1576 DOI: 10.1126/science.1347958
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Articles
Science, Vol 255, Issue 5051, 1573-1576
Copyright © 1992 by American Association for the Advancement of Science
Transcription factor loading on the MMTV promoter: a bimodal mechanism for promoter activation
TK Archer,
P Lefebvre,
RG Wolford,
and
GL Hager
Hormone Action and Oncogenesis Section, Laboratory of Molecular Virology, National Cancer Institute, Bethesda, MD 20892.
The mouse mammary tumor virus (MMTV) promoter attains a phased array of six nucleosomes when introduced into rodent cells. This architecture excludes nuclear factor 1/CCAAT transcription factor (NF1/CTF) from the promoter before glucocorticoid treatment and hormone-dependent access of nucleolytic agents to promoter DNA. In contrast, when the promoter was transiently introduced into cells, NF1/CTF was bound constitutively and nucleolytic attack was hormone-independent. Thus, induction at this promoter was a bimodal process involving receptor-dependent remodeling of chromatin that allows NF1/CTF loading and direct receptor-mediated recruitment of additional transcription factors.
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