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Science 13 March 1992:
Vol. 255. no. 5050, pp. 1425 - 1427
DOI: 10.1126/science.1542791
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Articles

Science, Vol 255, Issue 5050, 1425-1427
Copyright © 1992 by American Association for the Advancement of Science

articles

Suppression of gene amplification in human cell hybrids

TD Tlsty, A White, and J Sanchez

Lineberger Comprehensive Cancer Center, Department of Pathology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill 27599.

Gene amplification, one example of genetic instability, is of prognostic and clinical importance in neoplasia. In tumorigenic cells, gene amplification occurs at a very high frequency, whereas in normal diploid fibroblasts the event is undetectable by the clonogenic assay. To investigate genetic control of gene amplification, amplification frequency was measured in hybrids of tumorigenic cells and normal diploid cells. The ability to amplify an endogenous gene behaved as a recessive genetic trait, and control of gene amplification potential segregated independently of tumorigenicity and immortality.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Cancer Res. 60, 6496-6502
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Effects of Genome Position and the DNA Damage Checkpoint on the Structure and Frequency of sod2 Gene Amplification in Fission Yeast.
T. E. Patterson, E. B. Albrecht, P. Nurse, S. Sazer, and G. R. Stark (1999)
Mol. Biol. Cell 10, 2199-2208
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Deletion of p16INK4A/CDKN2 and p15INK4B in Human Somatic Cell Hybrids and Hybrid-derived Tumors.
S. J. Kuerbitz, J. Malandro, N. Compitello, S. B. Baylin, and J. R. Graff (1999)
Cell Growth Differ. 10, 27-33
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Differential disruption of genomic integrity and cell cycle regulation in normal human fibroblasts by the HPV oncoproteins..
A E White, E M Livanos, and T D Tlsty (1994)
Genes & Dev. 8, 666-677
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Genomic Integrity and the Genetics of Cancer.
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Cold Spring Harb Symp Quant Biol 59, 265-275
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Loss of Chromosomal Integrity in Neoplasia.
T.D. Tlsty, P. Jonczyk, A White, M. Sage, I. Hall, D. Schaefer, A. Briot, E. Livanos, H. Roelofs, B. Poulose, et al. (1993)
Cold Spring Harb Symp Quant Biol 58, 645-654
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Abstracts.
(1992)
Toxicol Pathol 20, 655-670
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