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Science 28 February 1992:
Vol. 255. no. 5048, pp. 1127 - 1129
DOI: 10.1126/science.1546313
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Articles

Science, Vol 255, Issue 5048, 1127-1129
Copyright © 1992 by American Association for the Advancement of Science

articles

Isolation of two genes that encode subunits of the yeast transcription factor IIA

JA Ranish, WS Lane, and S Hahn

Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

The yeast transcription factor IIA (TFIIA), a component of the basal transcription machinery of RNA polymerase II and implicated in vitro in regulation of basal transcription, is composed of two subunits of 32 and 13.5 kilodaltons. The genes that encode these subunits, termed TOA1 and TOA2, respectively, were cloned. Neither gene shares obvious sequence similarity with the other or with any other previously identified genes. The recombinant factor bound to a TATA binding protein-DNA complex and complemented yeast and mammalian in vitro transcription systems depleted of TFIIA. Both the TOA1 and TOA2 genes are essential for growth of yeast.


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Protease Footprinting Analysis of Ternary Complex Formation by Human TFIIA.
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The general transcription factors of RNA polymerase II..
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Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo..
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Transcription Factor IIA Mutations Show Activator-specific Defects and Reveal a IIA Function Distinct from Stimulation of TBP-DNA Binding.
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The TBP-TFIIA interaction in the response to acidic activators in vivo.
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Promoter-dependent Photocross-linking of the Acidic Transcriptional Activator E2F-1 to the TATA-binding Protein.
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Drosophila TFIIA directs cooperative DNA binding with TBP and mediates transcriptional activation..
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Molecular cloning of the small (gamma) subunit of human TFIIA reveals functions critical for activated transcription..
J Ozer, P A Moore, A H Bolden, A Lee, C A Rosen, and P M Lieberman (1994)
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Mot1, a global repressor of RNA polymerase II transcription, inhibits TBP binding to DNA by an ATP-dependent mechanism..
D T Auble, K E Hansen, C G Mueller, W S Lane, J Thorner, and S Hahn (1994)
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A mechanism for TAFs in transcriptional activation: activation domain enhancement of TFIID-TFIIA--promoter DNA complex formation..
P M Lieberman and A J Berk (1994)
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A single cDNA, hTFIIA/alpha, encodes both the p35 and p19 subunits of human TFIIA..
J DeJong and R G Roeder (1993)
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Drosophila TFIIA-L is processed into two subunits that are associated with the TBP/TAF complex..
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DNA melting on yeast RNA polymerase II promoters.
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A yeast TFIIB-related factor involved in RNA polymerase III transcription..
T Colbert and S Hahn (1992)
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Cloning of the 62-kilodalton component of basic transcription factor BTF2.
L Fischer, M Gerard, C Chalut, Y Lutz, S Humbert, M Kanno, P Chambon, and J. Egly (1992)
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TAFII 250 Phosphorylates Human Transcription Factor IIA on Serine Residues Important for TBP Binding and Transcription Activity.
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The Stability of the TFIIA-TBP-DNA Complex Is Dependent on the Sequence of the TATAAA Element.
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Fluorescence-based Analyses of the Effects of Full-length Recombinant TAF130p on the Interaction of TATA Box-binding Protein with TATA Box DNA.
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