Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 21 February 1992:
Vol. 255. no. 5047, pp. 983 - 986
DOI: 10.1126/science.1546295
Prev | Table of Contents | Next

Articles

Science, Vol 255, Issue 5047, 983-986
Copyright © 1992 by American Association for the Advancement of Science

articles

A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA

J Hagler and S Shuman

Program in Molecular Biology, Sloan-Kettering Institute, New York, NY 10021.

Ribonuclease footprinting of nascent messenger RNA within ternary complexes of vaccinia RNA polymerase revealed an RNA binding site that encompasses an 18-nucleotide RNA segment. The dimensions of the binding site did not change as the polymerase moved along the template. Capping of the 5' end of the RNA was cotranscriptional and was confined to nascent chains 31 nucleotides or greater in length. Purified capping enzyme formed a binary complex with RNA polymerase in solution in the absence of nucleic acid. These findings suggest a mechanism for cotranscriptional establishment of messenger RNA identity in eukaryotes.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Roles of LEF-4 and PTP/BVP RNA Triphosphatases in Processing of Baculovirus Late mRNAs.
Y. Li and L. A. Guarino (2008)
J. Virol. 82, 5573-5583
   Abstract »    Full Text »    PDF »
Exportin 1 Mediates Nuclear Export of the Kinetoplastid Spliced Leader RNA.
G. M. Zeiner, N. R. Sturm, and D. A. Campbell (2003)
Eukaryot. Cell 2, 222-230
   Abstract »    Full Text »    PDF »
UUUUUNU Oligonucleotide Stimulation of Vaccinia Virus Early Gene Transcription Termination, in Trans.
M. R. Mohamed and E. G. Niles (2003)
J. Biol. Chem. 278, 11794-11801
   Abstract »    Full Text »    PDF »
The mRNA Capping Apparatus as Drug Target and Guide to Eukaryotic Phylogeny.
S. SHUMAN (2001)
Cold Spring Harb Symp Quant Biol 66, 301-312
   Abstract »    PDF »
A Yeast-Based Genetic System for Functional Analysis of Viral mRNA Capping Enzymes.
C. K. Ho, A. Martins, and S. Shuman (2000)
J. Virol. 74, 5486-5494
   Abstract »    Full Text »
Transcription elongation factor hSPT5 stimulates mRNA capping.
Y. Wen and A. J. Shatkin (1999)
Genes & Dev. 13, 1774-1779
   Abstract »    Full Text »
Two Nucleotides Immediately Upstream of the Essential A6G3 Slippery Sequence Modulate the Pattern of G Insertions during Sendai Virus mRNA Editing.
S. Hausmann, D. Garcin, A.-S. Morel, and D. Kolakofsky (1999)
J. Virol. 73, 343-351
   Abstract »    Full Text »    PDF »
RNA 5'-Triphosphatase, Nucleoside Triphosphatase, and Guanylyltransferase Activities of Baculovirus LEF-4 Protein.
C. H. Gross and S. Shuman (1998)
J. Virol. 72, 10020-10028
   Abstract »    Full Text »    PDF »
Mammalian capping enzyme binds RNA and uses protein tyrosine phosphatase mechanism.
Y. Wen, Z. Yue, and A. J. Shatkin (1998)
PNAS 95, 12226-12231
   Abstract »    Full Text »    PDF »
Modifications of the 5' Cap of mRNAs during Xenopus Oocyte Maturation: Independence from Changes in Poly(A) Length and Impact on Translation.
D. L. Gillian-Daniel, N. K. Gray, J. Åström, A. Barkoff, and M. Wickens (1998)
Mol. Cell. Biol. 18, 6152-6163
   Abstract »    Full Text »
Recombinant Human mRNA Cap Methyltransferase Binds Capping Enzyme/RNA Polymerase IIo Complexes.
R. C. Pillutla, Z. Yue, E. Maldonado, and A. J. Shatkin (1998)
J. Biol. Chem. 273, 21443-21446
   Abstract »    Full Text »    PDF »
Vaccinia NPH-I, a DExH-box ATPase, is the energy coupling factor for mRNA transcription termination.
L. Deng and S. Shuman (1998)
Genes & Dev. 12, 538-546
   Abstract »    Full Text »
RNA Polymerase I-Promoted HIS4 Expression Yields Uncapped, Polyadenylated mRNA That Is Unstable and Inefficiently Translated in Saccharomyces cerevisiae.
H.-J. Lo, H.-K. Huang, and T. F. Donahue (1998)
Mol. Cell. Biol. 18, 665-675
   Abstract »    Full Text »
Promoter-associated Pausing in Promoter Architecture and Postinitiation Transcriptional Regulation.
J. LIS (1998)
Cold Spring Harb Symp Quant Biol 63, 347-356
   Abstract »    PDF »
Transcription units as RNA processing units.
K. M. Neugebauer and M. B. Roth (1997)
Genes & Dev. 11, 3279-3285
   Full Text »    PDF »
5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II.
S. McCracken, N. Fong, E. Rosonina, K. Yankulov, G. Brothers, D. Siderovski, A. Hessel, S. Foster, A. E. Program, S. Shuman, et al. (1997)
Genes & Dev. 11, 3306-3318
   Abstract »    Full Text »    PDF »
Drosophila Factor 2, an RNA Polymerase II Transcript Release Factor, Has DNA-dependent ATPase Activity.
Z. Xie and D. Price (1997)
J. Biol. Chem. 272, 31902-31907
   Abstract »    Full Text »    PDF »
Origins of mRNA identity: Capping enzymes bind to the phosphorylated C-terminal domain of RNA polymerase II.
S. Shuman (1997)
PNAS 94, 12758-12760
   Full Text »    PDF »
Mammalian capping enzyme complements mutant Saccharomyces cerevisiae lacking mRNA guanylyltransferase and selectively binds the elongating form of RNA polymerase II.
Z. Yue, E. Maldonado, R. Pillutla, H. Cho, D. Reinberg, and A. J. Shatkin (1997)
PNAS 94, 12898-12903
   Abstract »    Full Text »    PDF »
Factor-dependent Release of Nascent RNA by Ternary Complexes of Vaccinia RNA Polymerase.
L. Deng, J. Hagler, and S. Shuman (1996)
J. Biol. Chem. 271, 19556-19562
   Abstract »    Full Text »    PDF »
Characterization of the Vaccinia Virus RNA 5`-Triphosphatase and Nucleoside Triphosphate Phosphohydrolase Activities.
J. R. Myette and E. G. Niles (1996)
J. Biol. Chem. 271, 11945-11952
   Abstract »    Full Text »    PDF »
Transition from rapid processive to slow nonprocessive polyadenylation by vaccinia virus poly(A) polymerase catalytic subunit is regulated by the net length of the poly(A) tail..
P D Gershon and B Moss (1992)
Genes & Dev. 6, 1575-1586
   Abstract »    PDF »
Cotranscriptional Cap 4 Formation on the Trypanosoma brucei Spliced Leader RNA.
G. Mair, E. Ullu, and C. Tschudi (2000)
J. Biol. Chem. 275, 28994-28999
   Abstract »    Full Text »    PDF »
HIV-1 Tat Protein Interacts with Mammalian Capping Enzyme and Stimulates Capping of TAR RNA.
Y.-L. Chiu, E. Coronel, C. K. Ho, S. Shuman, and T. M. Rana (2001)
J. Biol. Chem. 276, 12959-12966
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)