Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 14 February 1992:
Vol. 255. no. 5046, pp. 841 - 845
DOI: 10.1126/science.1371365
Prev | Table of Contents | Next

Articles

Science, Vol 255, Issue 5046, 841-845
Copyright © 1992 by American Association for the Advancement of Science

articles

Mechanism of transduction by retroviruses

A Swain and JM Coffin

Tufts University School of Medicine, Department of Molecular Biology and Microbiology, Boston, MA 02111.

Retroviruses can capture cellular sequences and express them as oncogenes. Capture has been proposed to be a consequence of the inefficiency of polyadenylation of the viral genome that allows the packaging of cellular sequences flanking the integrated provirus in virions; after transfer into virions, these sequences could be incorporated into the viral genome by illegitimate recombination during reverse transcription. As a test for this hypothesis, a tissue culture system was developed that mimics the transduction process and allows the analysis and quantitation of capture events in a single step. In this model, transduction of sequences adjacent to a provirus depends on the formation of readthrough transcripts and their transmission in virions and leads to various recombinant structures whose formation is independent of sequence similarity at the crossover site. Thus, all events in the transduction process can be attributed to the action of reverse transcriptase on readthrough transcripts without involving deletions of cellular DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Darwinian evolution in the light of genomics.
E. V. Koonin (2009)
Nucleic Acids Res. 37, 1011-1034
   Abstract »    Full Text »    PDF »
Effects of Identity Minimization on Moloney Murine Leukemia Virus Template Recognition and Frequent Tertiary Template-Directed Insertions during Nonhomologous Recombination.
N. K. Duggal, L. Goo, S. R. King, and A. Telesnitsky (2007)
J. Virol. 81, 12156-12168
   Abstract »    Full Text »    PDF »
Epstein-Barr Virus Latent Membrane Protein LMP-2A Is Sufficient for Transactivation of the Human Endogenous Retrovirus HERV-K18 Superantigen.
N. Sutkowski, G. Chen, G. Calderon, and B. T. Huber (2004)
J. Virol. 78, 7852-7860
   Abstract »    Full Text »    PDF »
Human Immunodeficiency Virus Type 1 Transductive Recombination Can Occur Frequently and in Proportion to Polyadenylation Signal Readthrough.
W. An and A. Telesnitsky (2004)
J. Virol. 78, 3419-3428
   Abstract »    Full Text »    PDF »
Human Immunodeficiency Virus Type 1 Genetic Recombination Is More Frequent Than That of Moloney Murine Leukemia Virus despite Similar Template Switching Rates.
A. Onafuwa, W. An, N. D. Robson, and A. Telesnitsky (2003)
J. Virol. 77, 4577-4587
   Abstract »    Full Text »    PDF »
Transduction of Cellular Sequence by a Human Immunodeficiency Virus Type 1-Derived Vector.
G. Sun, P. K. O'Neil, H. Yu, Y. Ron, B. D. Preston, and J. P. Dougherty (2001)
J. Virol. 75, 11902-11906
   Abstract »    Full Text »
A Novel Hybrid Open Reading Frame Formed by Multiple Cellular Gene Transductions by a Plant Long Terminal Repeat Retroelement.
N. Elrouby and T. E. Bureau (2001)
J. Biol. Chem. 276, 41963-41968
   Abstract »    Full Text »    PDF »
Genomic Stability of Murine Leukemia Viruses Containing Insertions at the Env-3' Untranslated Region Boundary.
C. R. Logg, A. Logg, C.-K. Tai, P. M. Cannon, and N. Kasahara (2001)
J. Virol. 75, 6989-6998
   Abstract »    Full Text »
Effects of Homology Length in the Repeat Region on Minus-Strand DNA Transfer and Retroviral Replication.
Q. Dang and W.-S. Hu (2001)
J. Virol. 75, 809-820
   Abstract »    Full Text »
Utilization of Nonviral Sequences for Minus-Strand DNA Transfer and Gene Reconstitution during Retroviral Replication.
S. R. Cheslock, J. A. Anderson, C. K. Hwang, V. K. Pathak, and W.-S. Hu (2000)
J. Virol. 74, 9571-9579
   Abstract »    Full Text »
Poised for Contagion: Evolutionary Origins of the Infectious Abilities of Invertebrate Retroviruses.
H. S. Malik, S. Henikoff, and T. H. Eickbush (2000)
Genome Res. 10, 1307-1318
   Abstract »    Full Text »
Replication of Lengthened Moloney Murine Leukemia Virus Genomes Is Impaired at Multiple Stages.
N.-H. Shin, D. Hartigan-O'Connor, J. K. Pfeiffer, and A. Telesnitsky (2000)
J. Virol. 74, 2694-2702
   Abstract »    Full Text »
Most Retroviral Recombinations Occur during Minus-Strand DNA Synthesis.
J. Zhang, L.-Y. Tang, T. Li, Y. Ma, and C. M. Sapp (2000)
J. Virol. 74, 2313-2322
   Abstract »    Full Text »
Mutations of the Kissing-Loop Dimerization Sequence Influence the Site Specificity of Murine Leukemia Virus Recombination In Vivo.
J. G. Mikkelsen, A. H. Lund, M. Duch, and F. S. Pedersen (2000)
J. Virol. 74, 600-610
   Abstract »    Full Text »
Sequence and Transcriptional Analyses of the Fish Retroviruses Walleye Epidermal Hyperplasia Virus Types 1 and 2: Evidence for a Gene Duplication.
L. A. LaPierre, D. L. Holzschu, P. R. Bowser, and J. W. Casey (1999)
J. Virol. 73, 9393-9403
   Abstract »    Full Text »    PDF »
Point Mutations in the Avian Sarcoma/Leukosis Virus 3' Untranslated Region Result in a Packaging Defect.
J. M. Aschoff, D. Foster, and J. M. Coffin (1999)
J. Virol. 73, 7421-7429
   Abstract »    Full Text »
Genetic Analysis of the Rat Leukemia Virus: Influence of Viral Sequences in Transduction of the c-ras Proto-Oncogene and Expression of Its Transforming Activity.
S.-Y. Lee, T. M. Howard, and S. Rasheed (1998)
J. Virol. 72, 9906-9917
   Abstract »    Full Text »    PDF »
Walleye Retroviruses Associated with Skin Tumors and Hyperplasias Encode Cyclin D Homologs.
L. A. LaPierre, J. W. Casey, and D. L. Holzschu (1998)
J. Virol. 72, 8765-8771
   Abstract »    Full Text »    PDF »
Extreme Structural Heterogeneity Among the Members of a Maize Retrotransposon Family.
S. Marillonnet and S. R. Wessler (1998)
Genetics 150, 1245-1256
   Abstract »    Full Text »
An Array of Novel Murine Spleen Focus-Forming Viruses That Activate the Erythropoietin Receptor.
E. Gomez-Lucia, Y. Zhi, M. Nabavi, W. Zhang, D. Kabat, and M. E. Hoatlin (1998)
J. Virol. 72, 3742-3750
   Abstract »    Full Text »    PDF »
Extended Minus-Strand DNA as Template for R-U5-Mediated Second-Strand Transfer in Recombinational Rescue of Primer Binding Site-Modified Retroviral Vectors.
J. G. Mikkelsen, A. H. Lund, K. Dybkar, M. Duch, and F. S. Pedersen (1998)
J. Virol. 72, 2519-2525
   Abstract »    Full Text »    PDF »
High Fidelity of Internal Strand Transfer Catalyzed by Human Immunodeficiency Virus Reverse Transcriptase.
J. DeStefano, J. Ghosh, B. Prasad, and A. Raja (1998)
J. Biol. Chem. 273, 1483-1489
   Abstract »    Full Text »    PDF »
Rate and mechanism of nonhomologous recombination during a single cycle of retroviral replication.
J Zhang and H. Temin (1993)
Science 259, 234-238
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)