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Science 24 January 1992:
Vol. 255. no. 5043, pp. 453 - 456
DOI: 10.1126/science.1734522
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Articles

Science, Vol 255, Issue 5043, 453-456
Copyright © 1992 by American Association for the Advancement of Science

articles

DNA binding activity of recombinant SRY from normal males and XY females

VR Harley, DI Jackson, PJ Hextall, Hawkins JR, GD Berkovitz, S Sockanathan, R Lovell-Badge, and PN Goodfellow

Human Molecular Genetics Laboratory, Imperial Cancer Research Fund, London, United Kingdom.

The protein encoded by the human testis determining gene, SRY, contains a high mobility group (HMG) box related to that present in the T cell-specific, DNA-binding protein TCF-1. Recombinant SRY protein was able to bind to the same core sequence AACAAAG recognized by TCF-1 in a sequence dependent manner. In five XY females point mutations were found in the region encoding the HMG box. In four cases DNA binding activity of mutant SRY protein was negligible; in the fifth case DNA binding was reduced. These results imply that the DNA binding activity of SRY is required for sex determination.


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