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Science 17 January 1992:
Vol. 255. no. 5042, pp. 327 - 330
DOI: 10.1126/science.1549778
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Articles

Science, Vol 255, Issue 5042, 327-330
Copyright © 1992 by American Association for the Advancement of Science

articles

Cyclic 2',3'-phosphates and nontemplated nucleotides at the 3' end of spliceosomal U6 small nuclear RNA's

E Lund and JE Dahlberg

Department of Biomolecular Chemistry, University of Wisconsin Medical School, Madison 53706.

Spliceosomal U6 small nuclear RNA (U6 RNA) in species as diverse as man, frog, fruitfly, and soybean have at their 3' ends a cyclic 2',3'-phosphate (greater than p) apparently derived from uridylic acid residues that were added post-transcriptionally. The 3' ends of U6 RNA's from various sources may be processed in different ways, or to different extents, depending on the organism or stage of development. The presence of a greater than p terminus on U6 RNA may influence the activity of U6 RNA either directly during splicing or indirectly by ensuring that the RNA has a defined length or proper conformation (or both).


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