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Science 10 January 1992:
Vol. 255. no. 5041, pp. 195 - 197
DOI: 10.1126/science.1553545
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Articles

Science, Vol 255, Issue 5041, 195-197
Copyright © 1992 by American Association for the Advancement of Science

articles

Biological significance of unwinding capability of nuclear matrix-associating DNAs

J Bode, Y Kohwi, L Dickinson, T Joh, D Klehr, C Mielke, and T Kohwi-Shigematsu

Gesellschaft fur Biotechnologische Forschung, mbH, Genetik von Eukaryoten, Mascheroder Weg 1, Braunschweig-Stockheim, Federal Republic of Germany.

Matrix attachment regions (MARs) are thought to separate chromatin into topologically constrained loop domains. A MAR located 5' of the human beta-interferon gene becomes stably base-unpaired under superhelical strain, as do the MARs flanking the immunoglobulin heavy chain gene enhancer; in both cases a nucleation site exists for DNA unwinding. Concatemerized oligonucleotides containing the unwinding nucleation site exhibited a strong affinity for the nuclear scaffold and augmented SV40 promoter activity in stable transformants. Mutated concatemerized oligonucleotides resisted unwinding, showed weak affinity for the nuclear scaffold, and did not enhance promoter activity. These results suggest that the DNA feature capable of relieving superhelical strain is important for MAR functions.


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