Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 20 December 1991:
Vol. 254. no. 5039, pp. 1814 - 1816
DOI: 10.1126/science.1684878
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5039, 1814-1816
Copyright © 1991 by American Association for the Advancement of Science

articles

Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity

N Segil, SB Roberts, and N Heintz

Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.

Oct-1 is a transcription factor involved in the cell cycle regulation of histone H2B gene transcription and in the transcription of other cellular housekeeping genes. Oct-1 is hyperphosphorylated as cells enter mitosis, and mitosis-specific phosphorylation is reversed as cells exit mitosis. A mitosis-specific phosphorylation site in the homeodomain of Oct-1 was phosphorylated in vitro by protein kinase A. Phosphorylation of this site correlated with inhibition of Oct-1 DNA binding activity in vivo and in vitro. The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress.
J. Kang, M. Gemberling, M. Nakamura, F. G. Whitby, H. Handa, W. G. Fairbrother, and D. Tantin (2009)
Genes & Dev. 23, 208-222
   Abstract »    Full Text »    PDF »
Expression of the Muscle Atrophy Factor Muscle Atrophy F-Box Is Suppressed by Testosterone.
W. Zhao, J. Pan, X. Wang, Y. Wu, W. A. Bauman, and C. P. Cardozo (2008)
Endocrinology 149, 5449-5460
   Abstract »    Full Text »    PDF »
Histone 2B (H2B) Expression Is Confined to a Proper NAD+/NADH Redox Status.
R.-P. Dai, F.-X. Yu, S.-R. Goh, H.-W. Chng, Y.-L. Tan, J.-L. Fu, L. Zheng, and Y. Luo (2008)
J. Biol. Chem. 283, 26894-26901
   Abstract »    Full Text »    PDF »
Cell-cycle-specific nestin expression coordinates with morphological changes in embryonic cortical neural progenitors.
T. Sunabori, A. Tokunaga, T. Nagai, K. Sawamoto, M. Okabe, A. Miyawaki, Y. Matsuzaki, T. Miyata, and H. Okano (2008)
J. Cell Sci. 121, 1204-1212
   Abstract »    Full Text »    PDF »
Atorvastatin decreases lipoprotein lipase and endothelial lipase expression in human THP-1 macrophages.
G. Qiu and J. S. Hill (2007)
J. Lipid Res. 48, 2112-2122
   Abstract »    Full Text »    PDF »
The Protein Kinase C Pathway Acts through Multiple Transcription Factors to Repress Gonadotropin-Releasing Hormone Gene Expression in Hypothalamic GT1-7 Neuronal Cells.
Q. Tang, M. Mazur, and P. L. Mellon (2005)
Mol. Endocrinol. 19, 2769-2779
   Abstract »    Full Text »    PDF »
Repression of the prolactin promoter: a functional consequence of the heterodimerization between Pit-1 and Pit-1 {beta}.
R A Sporici, J S Hodskins, D M Locasto, L B Meszaros, A L Ferry, A M Weidner, C A Rinehart, J C Bailey, I M Mains, and S E Diamond (2005)
J. Mol. Endocrinol. 35, 317-331
   Abstract »    Full Text »    PDF »
The POU Transcription Factor Oct-1 Represses Virus-Induced Interferon A Gene Expression.
T. Mesplede, M.-L. Island, N. Christeff, F. Petek, J. Doly, and S. Navarro (2005)
Mol. Cell. Biol. 25, 8717-8731
   Abstract »    Full Text »    PDF »
Estrogens and Progesterone Promote Persistent CCND1 Gene Activation during G1 by Inducing Transcriptional Derepression via c-Jun/c-Fos/Estrogen Receptor (Progesterone Receptor) Complex Assembly to a Distal Regulatory Element and Recruitment of Cyclin D1 to Its Own Gene Promoter.
L. Cicatiello, R. Addeo, A. Sasso, L. Altucci, V. B. Petrizzi, R. Borgo, M. Cancemi, S. Caporali, S. Caristi, C. Scafoglio, et al. (2004)
Mol. Cell. Biol. 24, 7260-7274
   Abstract »    Full Text »    PDF »
Reduction in DNA-binding affinity of Cys2His2 zinc finger proteins by linker phosphorylation.
D. Jantz and J. M. Berg (2004)
PNAS 101, 7589-7593
   Abstract »    Full Text »    PDF »
Brn-2 Expression Controls Melanoma Proliferation and Is Directly Regulated by {beta}-Catenin.
J. Goodall, S. Martinozzi, T. J. Dexter, D. Champeval, S. Carreira, L. Larue, and C. R. Goding (2004)
Mol. Cell. Biol. 24, 2915-2922
   Abstract »    Full Text »    PDF »
STAT5 and Oct-1 Form a Stable Complex That Modulates Cyclin D1 Expression.
S. Magne, S. Caron, M. Charon, M.-C. Rouyez, and I. Dusanter-Fourt (2003)
Mol. Cell. Biol. 23, 8934-8945
   Abstract »    Full Text »    PDF »
Oct-1 Is Posttranslationally Modified and Exhibits Reduced Capacity To Bind Cognate Sites at Late Times after Infection with Herpes Simplex Virus 1.
S. J. Advani, L. O. Durand, R. R. Weichselbaum, and B. Roizman (2003)
J. Virol. 77, 11927-11932
   Abstract »    Full Text »    PDF »
Down-Regulation of Histone H2B by DNA-Dependent Protein Kinase in Response to DNA Damage through Modulation of Octamer Transcription Factor 1.
C. Schild-Poulter, A. Shih, N. C. Yarymowich, and R. J. G. Hache (2003)
Cancer Res. 63, 7197-7205
   Abstract »    Full Text »    PDF »
The Evolution of Transcriptional Regulation in Eukaryotes.
G. A. Wray, M. W. Hahn, E. Abouheif, J. P. Balhoff, M. Pizer, M. V. Rockman, and L. A. Romano (2003)
Mol. Biol. Evol. 20, 1377-1419
   Abstract »    Full Text »    PDF »
Mitotic and Stress-induced Phosphorylation of HsPI3K-C2{alpha} Targets the Protein for Degradation.
S. A. Didichenko, C. M. Fragoso, and M. Thelen (2003)
J. Biol. Chem. 278, 26055-26064
   Abstract »    Full Text »    PDF »
Cellular diversity in the developing nervous system: a temporal view from Drosophila.
T. Brody and W. F. Odenwald (2003)
Development 129, 3763-3770
   Abstract »    Full Text »    PDF »
Oct-1 Is Involved in the Transcriptional Repression of the Gonadotropin-Releasing Hormone Receptor Gene.
C. K. Cheng, C. M. Yeung, R. L. C. Hoo, B. K. C. Chow, and P. C. K. Leung (2002)
Endocrinology 143, 4693-4701
   Abstract »    Full Text »    PDF »
A common mechanism for mitotic inactivation of C2H2 zinc finger DNA-binding domains.
S. Dovat, T. Ronni, D. Russell, R. Ferrini, B. S. Cobb, and S. T. Smale (2002)
Genes & Dev. 16, 2985-2990
   Abstract »    Full Text »    PDF »
Mitotic Phosphorylation of Chromosomal Protein HMGN1 Inhibits Nuclear Import and Promotes Interaction with 14.3.3 Proteins.
M. Prymakowska-Bosak, R. Hock, F. Catez, J.-H. Lim, Y. Birger, H. Shirakawa, K. Lee, and M. Bustin (2002)
Mol. Cell. Biol. 22, 6809-6819
   Abstract »    Full Text »    PDF »
Episodic Activation of the Rat GnRH Promoter: Role of the Homeoprotein Oct-1.
R. Vazquez-Martinez, G. M. Leclerc, M. E. Wierman, and F. R. Boockfor (2002)
Mol. Endocrinol. 16, 2093-2100
   Abstract »    Full Text »    PDF »
The Paired-like Homeodomain Protein, Arix, Mediates Protein Kinase A-stimulated Dopamine beta -Hydroxylase Gene Transcription through Its Phosphorylation Status.
M. Adachi and E. J. Lewis (2002)
J. Biol. Chem. 277, 22915-22924
   Abstract »    Full Text »    PDF »
Histone Deacetylase Activity Represses Gamma Interferon-Inducible HLA-DR Gene Expression following the Establishment of a DNase I-Hypersensitive Chromatin Conformation.
A. Osborne, H. Zhang, W.-M. Yang, E. Seto, and G. Blanck (2001)
Mol. Cell. Biol. 21, 6495-6506
   Abstract »    Full Text »    PDF »
Mitotic Phosphorylation Prevents the Binding of HMGN Proteins to Chromatin.
M. Prymakowska-Bosak, T. Misteli, J. E. Herrera, H. Shirakawa, Y. Birger, S. Garfield, and M. Bustin (2001)
Mol. Cell. Biol. 21, 5169-5178
   Abstract »    Full Text »    PDF »
HIRA, the Human Homologue of Yeast Hir1p and Hir2p, Is a Novel Cyclin-cdk2 Substrate Whose Expression Blocks S-Phase Progression.
C. Hall, D. M. Nelson, X. Ye, K. Baker, J. A. DeCaprio, S. Seeholzer, M. Lipinski, and P. D. Adams (2001)
Mol. Cell. Biol. 21, 1854-1865
   Abstract »    Full Text »
POU Domain Factors in the Neuroendocrine System: Lessons from Developmental Biology Provide Insights into Human Disease.
B. Andersen and M. G. Rosenfeld (2001)
Endocr. Rev. 22, 2-35
   Abstract »    Full Text »
Serine Phosphorylation of Focal Adhesion Kinase in Interphase and Mitosis: A Possible Role in Modulating Binding to p130Cas.
A. Ma, A. Richardson, E. M. Schaefer, and J. T. Parsons (2001)
Mol. Biol. Cell 12, 1-12
   Abstract »    Full Text »
The LIM/Homeodomain Protein Islet-1 Modulates Estrogen Receptor Functions.
F. Gay, I. Anglade, Z. Gong, and G. Salbert (2000)
Mol. Endocrinol. 14, 1627-1648
   Abstract »    Full Text »
NPAT links cyclin E-Cdk2 to the regulation of replication-dependent histone gene transcription.
J. Zhao, B. K. Kennedy, B. D. Lawrence, D. A. Barbie, A. G. Matera, J. A. Fletcher, and E. Harlow (2000)
Genes & Dev. 14, 2283-2297
   Abstract »    Full Text »
Cell cycle-regulated phosphorylation of p220NPAT by cyclin E/Cdk2 in Cajal bodies promotes histone gene transcription.
T. Ma, B. A. Van Tine, Y. Wei, M. D. Garrett, D. Nelson, P. D. Adams, J. Wang, J. Qin, L. T. Chow, and J. W. Harper (2000)
Genes & Dev. 14, 2298-2313
   Abstract »    Full Text »
Mitotic Transcription Repression In Vivo in the Absence of Nucleosomal Chromatin Condensation.
C. A. Spencer, M. J. Kruhlak, H. L. Jenkins, X. Sun, and D. P. Bazett-Jones (2000)
J. Cell Biol. 150, 13-26
   Abstract »    Full Text »    PDF »
Transcription Factors Oct-1 and C/EBP{beta} (CCAAT/Enhancer-Binding Protein-{beta}) Are Involved in the Glutamate/Nitric Oxide/cyclic-Guanosine 5'-Monophosphate-Mediated Repression of Gonadotropin-Releasing Hormone Gene Expression.
D. D. Belsham and P. L. Mellon (2000)
Mol. Endocrinol. 14, 212-228
   Abstract »    Full Text »
Transactivation of Herpes Simplex Virus Type 1 Immediate-Early Gene Expression by Virion-Associated Factors Is Blocked by an Inhibitor of Cyclin-Dependent Protein Kinases.
R. Jordan, L. Schang, and P. A. Schaffer (1999)
J. Virol. 73, 8843-8847
   Abstract »    Full Text »    PDF »
Functional Interaction between Oct-1 and Retinoid X Receptor.
T. Kakizawa, T. Miyamoto, K. Ichikawa, A. Kaneko, S. Suzuki, M. Hara, T. Nagasawa, T. Takeda, J.-i. Mori, M. Kumagai, et al. (1999)
J. Biol. Chem. 274, 19103-19108
   Abstract »    Full Text »    PDF »
Retinoblastoma Protein Expression Leads to Reduced Oct-1 DNA Binding Activity and Enhances Interleukin-8 Expression.
H. Zhang, A. T. Shepherd, D. D. Eason, S. Wei, J. I. Diaz, J. Y. Djeu, G. D. Wu, and G. Blanck (1999)
Cell Growth Differ. 10, 457-465
   Abstract »    Full Text »
Temporal Activation of the Sea Urchin Late H1 Gene Requires Stage-Specific Phosphorylation of the Embryonic Transcription Factor SSAP.
Z. Li and G. Childs (1999)
Mol. Cell. Biol. 19, 3684-3695
   Abstract »    Full Text »    PDF »
Expression Cloning and Characterization of PREB (Prolactin Regulatory Element Binding), a Novel WD Motif DNA-Binding Protein with a Capacity to Regulate Prolactin Promoter Activity.
M. S. Fliss, P. M. Hinkle, and C. Bancroft (1999)
Mol. Endocrinol. 13, 644-657
   Abstract »    Full Text »
Jun Kinase Phosphorylates and Regulates the DNA Binding Activity of an Octamer Binding Protein, T-Cell Factor beta 1.
S. Kasibhatla, P. Tailor, N. Bonefoy-Berard, T. Mustelin, A. Altman, and A. Fotedar (1999)
Mol. Cell. Biol. 19, 2021-2031
   Abstract »    Full Text »    PDF »
The Glucocorticoid Receptor Is Tethered to DNA-bound Oct-1 at the Mouse Gonadotropin-releasing Hormone Distal Negative Glucocorticoid Response Element.
U. R. Chandran, B. S. Warren, C. T. Baumann, G. L. Hager, and D. B. DeFranco (1999)
J. Biol. Chem. 274, 2372-2378
   Abstract »    Full Text »    PDF »
The mitotically phosphorylated form of the transcription termination factor TTF-1 is associated with the repressed rDNA transcription machinery.
V Sirri, P Roussel, and D Hernandez-Verdun (1999)
J. Cell Sci. 112, 3259-3268
   Abstract »    PDF »
Identification of the In Vivo Casein Kinase II Phosphorylation Site within the Homeodomain of the Cardiac Tisue-Specifying Homeobox Gene Product Csx/Nkx2.5.
H. Kasahara and S. Izumo (1999)
Mol. Cell. Biol. 19, 526-536
   Abstract »    Full Text »    PDF »
New POU dimer configuration mediates antagonistic control of an osteopontin preimplantation enhancer by Oct-4 and Sox-2.
V. Botquin, H. Hess, G. Fuhrmann, C. Anastassiadis, M. K. Gross, G. Vriend, and H. R. Schöler (1998)
Genes & Dev. 12, 2073-2090
   Abstract »    Full Text »
Transcriptional Regulation of Interleukin-1beta Gene by Interleukin-1beta Itself Is Mediated in Part by Oct-1 in Thymic Stromal Cells.
Y.-H. Tseng and L. A. Schuler (1998)
J. Biol. Chem. 273, 12633-12641
   Abstract »    Full Text »    PDF »
Adenovirus Type 12-Induced Fragility of the Human RNU2 Locus Requires p53 Function.
Z. Li, A. Yu, and A. M. Weiner (1998)
J. Virol. 72, 4183-4191
   Abstract »    Full Text »    PDF »
Dissociation of Oct-1 from the Nuclear Peripheral Structure Induces the Cellular Aging-associated Collagenase Gene Expression.
S.-i. Imai, S. Nishibayashi, K. Takao, M. Tomifuji, T. Fujino, M. Hasegawa, and T. Takano (1997)
Mol. Biol. Cell 8, 2407-2419
   Abstract »    Full Text »
The Cyclin-dependent Kinase-activating Kinase (CAK) Assembly Factor, MAT1, Targets and Enhances CAK Activity on the POU Domains of Octamer Transcription Factors.
S. Inamoto, N. Segil, Z.-Q. Pan, M. Kimura, and R. G. Roeder (1997)
J. Biol. Chem. 272, 29852-29858
   Abstract »    Full Text »    PDF »
Considerations of transcriptional control mechanisms: Do TFIID-core promoter complexes recapitulate nucleosome-like functions?.
A. Hoffmann, T. Oelgeschlager, and R. G. Roeder (1997)
PNAS 94, 8928-8935
   Abstract »    Full Text »    PDF »
Functions of the POU domain genes Skn-1a/i and Tst-1/Oct-6/SCIP in epidermal differentiation..
B Andersen, W C Weinberg, O Rennekampff, R J McEvilly, J R Bermingham, F Hooshmand, V Vasilyev, J F Hansbrough, M R Pittelkow, S H Yuspa, et al. (1997)
Genes & Dev. 11, 1873-1884
   Abstract »    PDF »
Mitogenic stimulation of resting T cells causes rapid phosphorylation of the transcription factor LSF and increased DNA-binding activity..
J L Volker, L E Rameh, Q Zhu, J DeCaprio, and U Hansen (1997)
Genes & Dev. 11, 1435-1446
   Abstract »    PDF »
POU domain family values: flexibility, partnerships, and developmental codes..
A K Ryan and M G Rosenfeld (1997)
Genes & Dev. 11, 1207-1225
   PDF »
Target-dependent Effect of Phosphorylation on the DNA Binding Activity of the TAL1/SCL Oncoprotein.
K. S.S. Prasad and S. J. Brandt (1997)
J. Biol. Chem. 272, 11457-11462
   Abstract »    Full Text »    PDF »
Cell cycle-dependent phosphorylation of mammalian protein phosphatase 1 by cdc2 kinase.
Y.-G. Kwon, S. Y. Lee, Y. Choi, P. Greengard, and A. C. Nairn (1997)
PNAS 94, 2168-2173
   Abstract »    Full Text »    PDF »
Structure of Pit-1 POU domain bound to DNA as a dimer: unexpected arrangement and flexibility..
E M Jacobson, P Li, A Leon-del-Rio, M G Rosenfeld, and A K Aggarwal (1997)
Genes & Dev. 11, 198-212
   Abstract »    PDF »
Mitotic regulation of TFIID: inhibition of activator-dependent transcription and changes in subcellular localization..
N Segil, M Guermah, A Hoffmann, R G Roeder, and N Heintz (1996)
Genes & Dev. 10, 2389-2400
   Abstract »    PDF »
Differential Regulation of c-Myb-induced Transcription Activation by a Phosphorylation Site in the Negative Regulatory Domain.
M. R. Miglarese, A. F. Richardson, N. Aziz, and T. P. Bender (1996)
J. Biol. Chem. 271, 22697-22705
   Abstract »    Full Text »    PDF »
Identification of the Nuclear Localization Signal of the POU Domain Protein Tst-1/Oct6.
E. Sock, J. Enderich, M. G. Rosenfeld, and M. Wegner (1996)
J. Biol. Chem. 271, 17512-17518
   Abstract »    Full Text »    PDF »
Octamer Binding Factors and Their Coactivator Can Activate the Murine PU.1 (spi-1) Promoter.
H.-m. Chen, P. Zhang, H. S. Radomska, C. J. Hetherington, D.-E. Zhang, and D. G. Tenen (1996)
J. Biol. Chem. 271, 15743-15752
   Abstract »    Full Text »    PDF »
Neither the homeodomain nor the activation domain of Bicoid is specifically required for its down-regulation by the Torso receptor tyrosine kinase cascade.
Y Bellaiche, R Bandyopadhyay, C Desplan, and N Dostatni (1996)
Development 122, 3499-3508
   Abstract »    PDF »
Cell Cycle Regulation of a Novel DNA Binding Complex in Saccharomyces cerevisiae with E2F-like Properties.
S. Vemu and R. R. Reichel (1995)
J. Biol. Chem. 270, 20724-20729
   Abstract »    Full Text »    PDF »
The POU domain: versatility in transcriptional regulation by a flexible two-in-one DNA-binding domain..
W Herr and M A Cleary (1995)
Genes & Dev. 9, 1679-1693
   PDF »
Jun family members are controlled by a calcium-regulated, cyclosporin A-sensitive signaling pathway in activated T lymphocytes..
K S Ullman, J P Northrop, A Admon, and G R Crabtree (1993)
Genes & Dev. 7, 188-196
   Abstract »    PDF »
Cell Cycle-regulated Phosphorylation of the Human SIX1 Homeodomain Protein.
H. L. Ford, E. Landesman-Bollag, C. S. Dacwag, P. T. Stukenberg, A. B. Pardee, and D. C. Seldin (2000)
J. Biol. Chem. 275, 22245-22254
   Abstract »    Full Text »    PDF »
Oct-1 Preferentially Interacts with Androgen Receptor in a DNA-dependent Manner That Facilitates Recruitment of SRC-1.
M. I. Gonzalez and D. M. Robins (2001)
J. Biol. Chem. 276, 6420-6428
   Abstract »    Full Text »    PDF »
Regulation of Global Acetylation in Mitosis through Loss of Histone Acetyltransferases and Deacetylases from Chromatin.
M. J. Kruhlak, M. J. Hendzel, W. Fischle, N. R. Bertos, S. Hameed, X.-J. Yang, E. Verdin, and D. P. Bazett-Jones (2001)
J. Biol. Chem. 276, 38307-38319
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)