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Science 20 December 1991:
Vol. 254. no. 5039, pp. 1808 - 1810
DOI: 10.1126/science.1722352
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Articles

Science, Vol 254, Issue 5039, 1808-1810
Copyright © 1991 by American Association for the Advancement of Science

articles

Reverse transcriptase encoded by a human transposable element

SL Mathias, AF Scott, HH Kazazian Jr, JD Boeke, and A Gabriel

Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

L1 elements are highly repeated mammalian DNA sequences whose structure suggests dispersal by retrotransposition. A consensus L1 element encodes a protein with sequence similarity to known reverse transcriptases. The second open reading frame from the human L1 element L1.2A was expressed as a fusion protein targeted to Ty1 virus-like particles in Saccharomyces cerevisiae and shown to have reverse transcriptase activity. This activity was eliminated by a missense mutation in the highly conserved amino acid motif Y/F-X-D-D. Thus, L1 represents a potential source of the reverse transcriptase activity necessary for dispersion of the many classes of mammalian retroelements.


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Active Alu Element "A-Tails": Size Does Matter.
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CORE-SINEs: Eukaryotic short interspersed retroposing elements with common sequence motifs.
N. Gilbert and D. Labuda (1999)
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Exon Shuffling by L1 Retrotransposition.
J. V. Moran, R. J. DeBerardinis, and H. H. Kazazian Jr. (1999)
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Activation of the Mitogen-Activated Protein Kinase Cascade Is Necessary But Not Sufficient for Basic Fibroblast Growth Factor- and Epidermal Growth Factor-Stimulated Expression of Endothelial Nitric Oxide Synthase in Ovine Fetoplacental Artery Endothelial Cells.
J. Zheng, I. M. Bird, A. N. Melsaether, and R. R. Magness (1999)
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   Abstract »    Full Text »
Hybrid Ty1/HIV-1 elements used to detect inhibitors and monitor the activity of HIV-1 reverse transcriptase.
D. V. Nissley, P. L. Boyer, D. J. Garfinkel, S. H. Hughes, and J. N. Strathern (1998)
PNAS 95, 13905-13910
   Abstract »    Full Text »    PDF »
Interactions between Ty1 Retrotransposon RNA and the T and D Regions of the tRNAiMet Primer Are Required for Initiation of Reverse Transcription In Vivo.
S. Friant, T. Heyman, A. S. Byström, M. Wilhelm, and F. X. Wilhelm (1998)
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Evidence for Copurification of HERV-K-Related Transcripts and a Reverse Transcriptase Activity in Human Platelets From Patients With Essential Thrombocythemia.
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Blood 90, 4022-4030
   Abstract »    Full Text »    PDF »
In vitro properties of the first ORF protein from mouse LINE-1 support its role in ribonucleoprotein particle formation during retrotransposition.
V. O. Kolosha and S. L. Martin (1997)
PNAS 94, 10155-10160
   Abstract »    Full Text »    PDF »
DNA ``Fossils'' and Phylogenetic Analysis.
A. V. Furano and K. Usdin (1995)
J. Biol. Chem. 270, 25301-25304
   Full Text »    PDF »
Unusual Reverse Transcriptases.
M. F. Singer and M. F. Singer (1995)
J. Biol. Chem. 270, 24623-24626
   Full Text »    PDF »
Isolation of an active human transposable element.
B. Dombroski, S. Mathias, E Nanthakumar, A. Scott, and H. Kazazian Jr (1991)
Science 254, 1805-1808
   Abstract »    PDF »


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