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Science 20 December 1991:
Vol. 254. no. 5039, pp. 1779 - 1782
DOI: 10.1126/science.1837174
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Articles

Science, Vol 254, Issue 5039, 1779-1782
Copyright © 1991 by American Association for the Advancement of Science

articles

Novel fold and putative receptor binding site of granulocyte-macrophage colony-stimulating factor

K Diederichs, T Boone, and PA Karplus

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.

Granulocyte-macrophage colony-stimulating factor (GM-CSF) stimulates the development of and the cytotoxic activity of white blood cells. Recombinant human GM-CSF has proven useful in the treatment of blood disorders. The structure of GM-CSF, which was determined at 2.4 angstrom resolution by x-ray crystallography, has a novel fold combining a two-stranded antiparallel beta sheet with an open bundle of four alpha helices. Residues implicated in receptor recognition, which are distant in the primary sequence, are on adjacent alpha helices in the folded protein. A working model for the receptor binding site is presented.


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