Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 6 December 1991:
Vol. 254. no. 5037, pp. 1509 - 1512
DOI: 10.1126/science.1962213
Prev | Table of Contents | Next

Articles

Science, Vol 254, Issue 5037, 1509-1512
Copyright © 1991 by American Association for the Advancement of Science

articles

Systemic delivery of human growth hormone by injection of genetically engineered myoblasts

J Dhawan, LC Pan, GK Pavlath, MA Travis, AM Lanctot, and HM Blau

Department of Pharmacology, Stanford University School of Medicine, CA 94305.

A recombinant gene encoding human growth hormone (hGH) was stably introduced into cultured myoblasts with a retroviral vector. After injection of genetically engineered myoblasts into mouse muscle, hGH could be detected in serum for 3 months. The fate of injected myoblasts was assessed by coinfecting the cells with two retroviral vectors, one encoding hGH and the other encoding beta-galactosidase from Escherichia coli. These results provide evidence that myoblasts, which can fuse into preexisting multinucleated myofibers that are vascularized and innervated, may be advantageous as vehicles for systemic delivery of recombinant proteins.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors.
A. Sacco, R. Doyonnas, M. A. LaBarge, M. M. Hammer, P. Kraft, and H. M. Blau (2005)
J. Cell Biol. 171, 483-492
   Abstract »    Full Text »    PDF »
Gene therapy of pituitary diseases.
E. J. Lee and J L. Jameson (2005)
J. Endocrinol. 185, 353-362
   Abstract »    Full Text »    PDF »
Gene Therapy for Central Diabetes Insipidus: Effective Antidiuresis by Muscle-Targeted Gene Transfer.
M. Yoshida, Y. Iwasaki, M. Asai, T. Nigawara, and Y. Oiso (2004)
Endocrinology 145, 261-268
   Abstract »    Full Text »    PDF »
Gene Therapy in Orthopaedic Surgery.
D. Hannallah, B. Peterson, J. R. Lieberman, F. H. Fu, and J. Huard (2002)
J. Bone Joint Surg. Am. 84, 1046-1061
   Full Text »
Recombinant Vascular Endothelial Growth Factor Secreted From Tissue-Engineered Bioartificial Muscles Promotes Localized Angiogenesis.
Y. Lu, J. Shansky, M. Del Tatto, P. Ferland, X. Wang, and H. Vandenburgh (2001)
Circulation 104, 594-599
   Abstract »    Full Text »    PDF »
Posttranslational modifications of recombinant myotube-synthesized human factor IX.
V. R. Arruda, J. N. Hagstrom, J. Deitch, T. Heiman-Patterson, R. M. Camire, K. Chu, P. A. Fields, R. W. Herzog, L. B. Couto, P. J. Larson, et al. (2001)
Blood 97, 130-138
   Abstract »    Full Text »    PDF »
VEGF Gene Delivery to Myocardium : Deleterious Effects of Unregulated Expression.
R. J. Lee, M. L. Springer, W. E. Blanco-Bose, R. Shaw, P. C. Ursell, and H. M. Blau (2000)
Circulation 102, 898-901
   Abstract »    Full Text »    PDF »
Distinct Families of Z-line Targeting Modules in the COOH-terminal Region of Nebulin.
K. Ojima, Z.X. Lin, M.-L. Bang, S. Holtzer, R. Matsuda, S. Labeit, H.L. Sweeney, and H. Holtzer (2000)
J. Cell Biol. 150, 553-566
   Abstract »    Full Text »    PDF »
Cell Transplantation as Future Therapy for Cardiovascular Disease? : A Workshop of the National Heart, Lung, and Blood Institute.
L. Reinlib and L. Field (2000)
Circulation 101 , e182-e187
   Abstract »    Full Text »    PDF »
Kinetics of Recombinant Adeno-Associated Virus-Mediated Gene Transfer.
A. K. Malik, P. E. Monahan, D. L. Allen, B.-G. Chen, R. J. Samulski, and K. Kurachi (2000)
J. Virol. 74, 3555-3565
   Abstract »    Full Text »
Reduced amount of the glucose-regulated protein GRP94 in skeletal myoblasts results in loss of fusion competence.
L. GORZA and M. VITADELLO (2000)
FASEB J 14, 461-475
   Abstract »    Full Text »
Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell-like Properties as the Myogenic Source.
J. R. Beauchamp, J. E. Morgan, C. N. Pagel, and T. A. Partridge (1999)
J. Cell Biol. 144, 1113-1122
   Abstract »    Full Text »    PDF »
IGF-1 Gene Transfer Into Denervated Rat Laryngeal Muscle.
P. W. Flint, A. Shiotani, and B. W. O'Malley Jr (1999)
Arch Otolaryngol Head Neck Surg 125, 274-279
   Abstract »    Full Text »    PDF »
Graded transcriptional response to different concentrations of a single transactivator.
A. M. Kringstein, F. M. V. Rossi, A. Hofmann, and H. M. Blau (1998)
PNAS 95, 13670-13675
   Abstract »    Full Text »    PDF »
Control of Erythropoietin Delivery by Doxycycline in Mice After Intramuscular Injection of Adeno-Associated Vector.
D. Bohl, A. Salvetti, P. Moullier, and J. M. Heard (1998)
Blood 92, 1512-1517
   Abstract »    Full Text »    PDF »
Histamine Induces Nuclear Factor of Activated T Cell-Mediated Transcription and Cyclosporin A-Sensitive Interleukin-8 mRNA Expression in Human Umbilical Vein Endothelial Cells.
V. Boss, X. Wang, L. F. Koppelman, K. Xu, and T. J. Murphy (1998)
Mol. Pharmacol. 54, 264-272
   Abstract »    Full Text »
In Vitro Packaging of Adeno-Associated Virus DNA.
X. Zhou and N. Muzyczka (1998)
J. Virol. 72, 3241-3247
   Abstract »    Full Text »    PDF »
Prolonged Reduction of High Blood Pressure With Human Nitric Oxide Synthase Gene Delivery.
K.-F. Lin, L. Chao, and J. Chao (1997)
Hypertension 30, 307-313
   Abstract »    Full Text »
Transgenic studies with a keratin promoter-driven growth hormone transgene: Prospects for gene therapy.
X. Wang, S. Zinkel, K. Polonsky, and E. Fuchs (1997)
PNAS 94, 219-226
   Abstract »    Full Text »    PDF »
Angiogenic Gene Therapy: A Leg to Stand On?.
J. C. Fox and J. L. Swain (1996)
Circulation 94, 3065-3066
   Full Text »
Gene delivery to skeletal muscle results in sustained expression and systemic delivery of a therapeutic protein.
P. D. Kessler, G. M. Podsakoff, X. Chen, S. A. McQuiston, P. C. Colosi, L. A. Matelis, G. J. Kurtzman, and B. J. Byrne (1996)
PNAS 93, 14082-14087
   Abstract »    Full Text »    PDF »
Transport pathway, maturation, and targetting of the vesicular stomatitis virus glycoprotein in skeletal muscle fibers.
P Rahkila, A Alakangas, K Vaananen, and K Metsikko (1996)
J. Cell Sci. 109, 1585-1596
   Abstract »    PDF »
Muscle-Mediated Gene Therapy.
H. M. Blau and M. L. Springer (1995)
N. Engl. J. Med. 333, 1554-1556
   Full Text »    PDF »
Human Atrial Natriuretic Peptide Gene Delivery Reduces Blood Pressure in Hypertensive Rats.
K.-F. Lin, J. Chao, and L. Chao (1995)
Hypertension 26, 847-853
   Abstract »    Full Text »
Gene Therapy for Cardiovascular Disease.
E. G. Nabel (1995)
Circulation 91, 541-548
   Full Text »
Intramuscular Delivery of Rat Kallikrein-binding Protein Gene Reverses Hypotension in Transgenic Mice Expressing Human Tissue Kallikrein.
J.-x. Ma, Z. Yang, J. Chao, and L. Chao (1995)
J. Biol. Chem. 270, 451-455
   Abstract »    Full Text »    PDF »
Inhibition of myogenic differentiation in myoblasts expressing a truncated type II TGF-beta receptor.
E. Filvaroff, R Ebner, and R Derynck (1994)
Development 120, 1085-1095
   Abstract »    PDF »
Somatic Gene Therapy in Otolaryngology--Head and Neck Surgery.
B. W. O'Malley Jr and F. D. Ledley (1993)
Arch Otolaryngol Head Neck Surg 119, 1191-1197
   Abstract »    PDF »
Gene Therapy.
F. Levine and T. Friedmann (1993)
Arch Pediatr Adolesc Med 147, 1167-1174
   Abstract »    PDF »
Implanted myoblasts not only fuse with myofibers but also survive as muscle precursor cells.
S. Yao and K Kurachi (1993)
J. Cell Sci. 105, 957-963
   Abstract »    PDF »
Review: Experimental and Clinical Applications of Molecular Cell Biology in Nutrition and Metabolism.
Yuman Fong, L. L. Moldawer, and S. F. Lowry (1992)
JPEN J Parenter Enteral Nutr 16, 477-486
   Abstract »    PDF »
Human Gene Therapy: A Role for the Primary Care Physician.
C. J. Schmeichel and J. M. Loeb (1992)
Arch Fam Med 1, 113-120
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)