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Science 29 November 1991:
Vol. 254. no. 5036, pp. 1385 - 1387
DOI: 10.1126/science.1683715
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Articles

Science, Vol 254, Issue 5036, 1385-1387
Copyright © 1991 by American Association for the Advancement of Science

articles

Regulation of a segmentation stripe by overlapping activators and repressors in the Drosophila embryo

D Stanojevic, S Small, and M Levine

Department of Biological Sciences, Fairchild Center, Columbia University, New York, NY 10027.

Gene expression stripes in Drosophila melanogaster embryos provide a model for how eukaryotic promoters are turned on and off in response to combinations of transcriptional regulators. Genetic studies suggested that even-skipped (eve) stripe 2 is controlled by three gap genes, hunchback (hb), Kruppel (Kr), and giant (gt), and by the maternal morphogen bicoid (bcd). A direct link is established between binding sites for these regulatory proteins in the stripe 2 promoter element and the expression of the stripe during early embryogenesis. The bcd and hb protein binding sites mediate activation, whereas neighboring gt and Kr protein sites repress expression and establish the stripe borders. The stripe 2 element has the properties of a genetic on-off switch.


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