Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 November 1991:
Vol. 254. no. 5035, pp. 1194 - 1197
DOI: 10.1126/science.254.5035.1194
Prev | Table of Contents | Next

Articles

Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani

KAREN BROGUE 1, ILAN CHET 1, MARK HOLLIDAY 1, ROBERT CRESSMAN 1, PHYLLIS BIDDLE 1, SUSAN KNOWLTON 1, C. JEFFRY MAUVAIS 1, and RICHARD BROGLIE 1

1 Agricultural Products Department, E. I. du Pont de Nemours, Wilmington, DE 19880

The production of enzymes capable of degrading the cell walls of invading phytopathogenic fungi is an important component of the defense response of plants. The timing of this natural host defense mechanism was modified to produce fungal-resistant plants. Transgenic tobacco seedlings constitutively expressing a bean chitinase gene under control of the cauliflower mosaic virus 35S promoter showed an increased ability to survive in soil infested with the fungal pathogen Rhizoctonia solani and delayed development of disease symptoms.

Submitted on July 2, 1991
Accepted on September 16, 1991


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Involvement of the Pepper Antimicrobial Protein CaAMP1 Gene in Broad Spectrum Disease Resistance.
S. C. Lee, I. S. Hwang, H. W. Choi, and B. K. Hwang (2008)
Plant Physiology 148, 1004-1020
   Abstract »    Full Text »    PDF »
A new type of plant chitinase containing LysM domains from a fern (Pteris ryukyuensis): Roles of LysM domains in chitin binding and antifungal activity.
S. Onaga and T. Taira (2008)
Glycobiology 18, 414-423
   Abstract »    Full Text »    PDF »
A LysM Receptor-Like Kinase Plays a Critical Role in Chitin Signaling and Fungal Resistance in Arabidopsis.
J. Wan, X.-C. Zhang, D. Neece, K. M. Ramonell, S. Clough, S.-y. Kim, M. G. Stacey, and G. Stacey (2008)
PLANT CELL 20, 471-481
   Abstract »    Full Text »    PDF »
From the Academy: Colloquium Perspective: Toward cropping systems that enhance productivity and sustainability.
R. J. Cook (2006)
PNAS 103, 18389-18394
   Abstract »    Full Text »    PDF »
Rice chitinases: sugar recognition specificities of the individual subsites.
C. Sasaki, K. M. Varum, Y. Itoh, M. Tamoi, and T. Fukamizo (2006)
Glycobiology 16, 1242-1250
   Abstract »    Full Text »    PDF »
A Comprehensive Structure-Function Analysis of Arabidopsis SNI1 Defines Essential Regions and Transcriptional Repressor Activity.
R. A. Mosher, W. E. Durrant, D. Wang, J. Song, and X. Dong (2006)
PLANT CELL 18, 1750-1765
   Abstract »    Full Text »    PDF »
Rewiring Mitogen-Activated Protein Kinase Cascade by Positive Feedback Confers Potato Blight Resistance.
C. Yamamizo, K. Kuchimura, A. Kobayashi, S. Katou, K. Kawakita, J. D.G. Jones, N. Doke, and H. Yoshioka (2006)
Plant Physiology 140, 681-692
   Abstract »    Full Text »    PDF »
The Non-catalytic Chitin-binding Protein CBP21 from Serratia marcescens Is Essential for Chitin Degradation.
G. Vaaje-Kolstad, S. J. Horn, D. M. F. van Aalten, B. Synstad, and V. G. H. Eijsink (2005)
J. Biol. Chem. 280, 28492-28497
   Abstract »    Full Text »    PDF »
Microarray Analysis Reveals Vegetative Molecular Phenotypes of Arabidopsis Flowering-time Mutants.
I. W. Wilson, G. C. Kennedy, J. W. Peacock, and E. S. Dennis (2005)
Plant Cell Physiol. 46, 1190-1201
   Abstract »    Full Text »    PDF »
A Peroxiredoxin Q Homolog from Gentians is Involved in Both Resistance Against Fungal Disease and Oxidative Stress.
A. Kiba, M. Nishihara, N. Tsukatani, T. Nakatsuka, Y. Kato, and S. Yamamura (2005)
Plant Cell Physiol. 46, 1007-1015
   Abstract »    Full Text »    PDF »
Selection on Glycine {beta}-1,3-Endoglucanase Genes Differentially Inhibited by a Phytophthora Glucanase Inhibitor Protein.
J. G. Bishop, D. R. Ripoll, S. Bashir, C. M. B. Damasceno, J. D. Seeds, and J. K. C. Rose (2005)
Genetics 169, 1009-1019
   Abstract »    Full Text »    PDF »
An alternative agriculture system is defined by a distinct expression profile of select gene transcripts and proteins.
V. Kumar, D. J. Mills, J. D. Anderson, and A. K. Mattoo (2004)
PNAS 101, 10535-10540
   Abstract »    Full Text »    PDF »
Temporal and Spatial Profiles of Chitinase Expression by Norway Spruce in Response to Bark Colonization by Heterobasidion annosum.
A. M. Hietala, H. Kvaalen, A. Schmidt, N. Johnk, H. Solheim, and C. G. Fossdal (2004)
Appl. Envir. Microbiol. 70, 3948-3953
   Abstract »    Full Text »    PDF »
Comparative Evolutionary Histories of Chitinase Genes in the Genus Zea and Family Poaceae.
P. Tiffin (2004)
Genetics 167, 1331-1340
   Abstract »    Full Text »    PDF »
Distribution and Phylogenetic Analysis of Family 19 Chitinases in Actinobacteria.
T. Kawase, A. Saito, T. Sato, R. Kanai, T. Fujii, N. Nikaidou, K. Miyashita, and T. Watanabe (2004)
Appl. Envir. Microbiol. 70, 1135-1144
   Abstract »    Full Text »    PDF »
C-Terminal Domain of a Hevein-Like Protein from Wasabia japonica has Potent Antimicrobial Activity.
A. Kiba, H. Saitoh, M. Nishihara, K. Omiya, and S. Yamamura (2003)
Plant Cell Physiol. 44, 296-303
   Abstract »    Full Text »    PDF »
Greenhouse and field testing of transgenic wheat plants stably expressing genes for thaumatin-like protein, chitinase and glucanase against Fusarium graminearum.
A. Anand, T. Zhou, H. N. Trick, B. S. Gill, W. W. Bockus, and S. Muthukrishnan (2003)
J. Exp. Bot. 54, 1101-1111
   Abstract »    Full Text »    PDF »
An Antifungal Protein from the Marine Bacterium Streptomyces sp. Strain AP77 Is Specific for Pythium porphyrae, a Causative Agent of Red Rot Disease in Porphyra spp..
J.-H. Woo, E. Kitamura, H. Myouga, and Y. Kamei (2002)
Appl. Envir. Microbiol. 68, 2666-2675
   Abstract »    Full Text »    PDF »
Induced Systemic Resistance (ISR) Against Pathogens in the Context of Induced Plant Defences.
M. HEIL and R. M. BOSTOCK (2002)
Ann. Bot. 89, 503-512
   Abstract »    Full Text »    PDF »
A class I chitinase from soybean seed coat.
M. Gijzen, K. Kuflu, D. Qutob, and J. T. Chernys (2001)
J. Exp. Bot. 52, 2283-2289
   Abstract »    Full Text »    PDF »
Nuclear Localization of NPR1 Is Required for Activation of PR Gene Expression.
M. Kinkema, W. Fan, and X. Dong (2000)
PLANT CELL 12, 2339-2350
   Abstract »    Full Text »
Local and Systemic Induction of Two Defense-Related Subtilisin-Like Protease Promoters in Transgenic Arabidopsis Plants. Luciferin Induction of PR Gene Expression.
L. Jordá and P. Vera (2000)
Plant Physiology 124, 1049-1058
   Abstract »    Full Text »
Rapid evolution in plant chitinases: Molecular targets of selection in plant-pathogen coevolution.
J. G. Bishop, A. M. Dean, and T. Mitchell-Olds (2000)
PNAS 97, 5322-5327
   Abstract »    Full Text »    PDF »
Antimicrobial Peptides Protect Coho Salmon from Vibrio anguillarum Infections.
X. Jia, A. Patrzykat, R. H. Devlin, P. A. Ackerman, G. K. Iwama, and R. E. W. Hancock (2000)
Appl. Envir. Microbiol. 66, 1928-1932
   Abstract »    Full Text »
Chitinase Activity in Tall Fescue Seedlings as Affected by Cultivar, Seedling Development, and Ethephon.
S.M. Marek, C.A. Roberts, A.L. Karr, and D.A. Sleper (2000)
Crop Sci. 40, 713-716
   Abstract »    Full Text »
Pti4 Is Induced by Ethylene and Salicylic Acid, and Its Product Is Phosphorylated by the Pto Kinase.
Y.-Q. Gu, C. Yang, V. K. Thara, J. Zhou, and G. B. Martin (2000)
PLANT CELL 12, 771-786
   Abstract »    Full Text »
Response to Xanthomonas campestris pv. vesicatoria in Tomato Involves Regulation of Ethylene Receptor Gene Expression.
J. A. Ciardi, D. M. Tieman, S. T. Lund, J. B. Jones, R. E. Stall, and H. J. Klee (2000)
Plant Physiology 123, 81-92
   Abstract »    Full Text »
Anticipating Endoplasmic Reticulum Stress: A Novel Early Response before Pathogenesis-Related Gene Induction.
E. P. W. M. Jelitto-Van Dooren, S. Vidal, and J. Denecke (1999)
PLANT CELL 11, 1935-1944
   Abstract »    Full Text »
Pathogen-Induced Elicitin Production in Transgenic Tobacco Generates a Hypersensitive Response and Nonspecific Disease Resistance.
H. Keller, N. Pamboukdjian, M. Ponchet, A. Poupet, R. Delon, J.-L. Verrier, D. Roby, and P. Ricci (1999)
PLANT CELL 11, 223-236
   Abstract »    Full Text »
The molecular mechanisms responsible for resistance in plant-pathogen interactions of the gene-for-gene type function more broadly than previously imagined.
R. J. Cook (1998)
PNAS 95, 9711-9712
   Full Text »    PDF »
Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens.
M. Lorito, S. L. Woo, I. G. Fernandez, G. Colucci, G. E. Harman, J. A. Pintor-Toro, E. Filippone, S. Muccifora, C. B. Lawrence, A. Zoina, et al. (1998)
PNAS 95, 7860-7865
   Abstract »    Full Text »    PDF »
Isolation of New Arabidopsis Mutants With Enhanced Disease Susceptibility to Pseudomonas syringae by Direct Screening.
S. M. Volko, T. Boller, and F. M. Ausubel (1998)
Genetics 149, 537-548
   Abstract »    Full Text »    PDF »
The Molecular Basis of Quantitative Genetic Variation in Central and Secondary Metabolism in Arabidopsis.
T. Mitchell-Olds and D. Pedersen (1998)
Genetics 149, 739-747
   Abstract »    Full Text »    PDF »
Generation of broad-spectrum disease resistance by overexpression of an essential regulatory gene in systemic acquired resistance.
H. Cao, X. Li, and X. Dong (1998)
PNAS 95, 6531-6536
   Abstract »    Full Text »    PDF »
The role of enzyme distortion in the single displacement mechanism of family 19 chitinases.
K. A. Brameld and W. A. Goddard III (1998)
PNAS 95, 4276-4281
   Abstract »    Full Text »    PDF »
A Central Role of Salicylic Acid in Plant Disease Resistance.
T. P. Delaney, S. Uknes, B. Vernooij, L. Friedrich, K. Weymann, D. Negrotto, T. Gaffney, M. Gut-Rella, H. Kessmann, E. Ward, et al. (1994)
Science 266, 1247-1250
   Abstract »    PDF »
Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid.
Z Chen, H Silva, and D. Klessig (1993)
Science 262, 1883-1886
   Abstract »    PDF »
Improving Plant Disease Resistance.
A. S. Moffat (1992)
Science 257, 482-483
   PDF »
Tomato Transcription Factors Pti4, Pti5, and Pti6 Activate Defense Responses When Expressed in Arabidopsis.
Y.-Q. Gu, M. C. Wildermuth, S. Chakravarthy, Y.-T. Loh, C. Yang, X. He, Y. Han, and G. B. Martin (2002)
PLANT CELL 14, 817-831
   Abstract »    Full Text »    PDF »
Mutation of a Chitinase-Like Gene Causes Ectopic Deposition of Lignin, Aberrant Cell Shapes, and Overproduction of Ethylene.
R. Zhong, S. J. Kays, B. P. Schroeder, and Z.-H. Ye (2002)
PLANT CELL 14, 165-179
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)