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Science 15 November 1991:
Vol. 254. no. 5034, pp. 998 - 1000
DOI: 10.1126/science.254.5034.998
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Articles
Indole-3-Acetic Acid Biosynthesis in the Mutant Maize orange pericarp, a Tryptophan Auxotroph
ALLEN D. WRIGHT 1,
MICHAEL B. SAMPSON 1,
M. GERALD NEUFFER 1,
LECH MICHALCZUK 2,
JANET PERNISE SLOVIN 2, and
JERRY D. COHEN 3
1 Department of Agronomy, University of Missouri, Columbia, MO 65211
2 Department of Botany, University of Maryland, College Park, MD 20742
3 Plant Hormone Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, Department of Agriculture, Beltsville, MD 20705
The maize mutant orange pericarp is a tryptophan auxotroph, which results from mutation of two unlinked loci of tryptophan synthase B. This mutant was used to test the hypothesis that tryptophan is the precursor to the plant hormone indole-3-acetic acid (IAA). Total IAA in aseptically grown mutant seedlings was 50 times greater than in normal seedlings. In mutant seedlings grown on media containing stable isotopelabeled precursors, IAA was more enriched than was tryptophan. No incorporation of label into IAA from tryptophan could be detected. These results establish that IAA can be produced de novo without tryptophan as an intermediate.
Submitted on June 28, 1991
Accepted on September 30, 1991
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