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Science 15 November 1991:
Vol. 254. no. 5034, pp. 1022 - 1024
DOI: 10.1126/science.1658940
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Articles

Science, Vol 254, Issue 5034, 1022-1024
Copyright © 1991 by American Association for the Advancement of Science

articles

Expression cloning of an adenylate cyclase-coupled calcitonin receptor

HY Lin, TL Harris, MS Flannery, A Aruffo, EH Kaji, A Gorn, LF Kolakowski Jr, HF Lodish, and Goldring SR

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

A calcitonin receptor complementary DNA (cDNA) was cloned by expression of a cDNA library from a porcine kidney epithelial cell line in COS cells. The 482-amino acid receptor has high affinity for salmon calcitonin (dissociation constant Kd approximately 6 nM) and is functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP). The receptor shows no sequence similarity to other reported G protein-coupled receptors but is homologous to the parathyroid hormone-parathyroid hormone-related peptide (PTH-PTHrP) receptor, indicating that the receptors for these hormones, which regulate calcium homeostasis, represent a new family of G protein-coupled receptors.


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J. Biol. Chem. 271, 19888-19893
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Characterization of Deletion and Truncation Mutants of the Rat Glucagon Receptor.
C. G. Unson, A. M. Cypess, H. N. Kim, P. K. Goldsmith, C. J. L. Carruthers, R. B. Merrifield, and T. P. Sakmar (1995)
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Critical Contributions of Amino-terminal Extracellular Domains in Agonist Binding and Activation of Secretin and Vasoactive Intestinal Polypeptide Receptors.
M. H. Holtmann, E. M. Hadac, and L. J. Miller (1995)
J. Biol. Chem. 270, 14394-14398
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Truncation of the Carboxyl-terminal Region of the Rat Parathyroid Hormone (PTH)/PTH-related Peptide Receptor Enhances PTH Stimulation of Adenylyl Cyclase but Not Phospholipase C.
A. Iida-Klein, J. Guo, L. Y. Xie, H. Jüppner, J. T. Potts Jr., H. M. Kronenberg, F. R. Bringhurst, A. B. Abou-Samra, and G. V. Segre (1995)
J. Biol. Chem. 270, 8458-8465
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Expression cloning and signaling properties of the rat glucagon receptor.
L. Jelinek, S Lok, G. Rosenberg, R. Smith, F. Grant, S Biggs, P. Bensch, J. Kuijper, P. Sheppard, C. Sprecher, et al. (1993)
Science 259, 1614-1616
   Abstract »    PDF »
The cloning of a family of genes that encode the melanocortin receptors.
K. Mountjoy, L. Robbins, M. Mortrud, and R. Cone (1992)
Science 257, 1248-1251
   Abstract »    PDF »
Tissue-specific and Ubiquitous Promoters Direct the Expression of Alternatively Spliced Transcripts from the Calcitonin Receptor Gene.
O. Anusaksathien, C. Laplace, X. Li, Y. Ren, L. Peng, S. R. Goldring, and D. L. Galson (2001)
J. Biol. Chem. 276, 22663-22674
   Abstract »    Full Text »    PDF »


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