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Science 1 November 1991:
Vol. 254. no. 5032, pp. 703 - 706
DOI: 10.1126/science.1948048
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Articles

Science, Vol 254, Issue 5032, 703-706
Copyright © 1991 by American Association for the Advancement of Science

articles

Calcium gradients underlying polarization and chemotaxis of eosinophils

RA Brundage, KE Fogarty, RA Tuft, and FS Fay

Department of Physiology, University of Massachusetts Medical School, Worcester 01605.

The concentration of intracellular free calcium ([Ca2+]i) in polarized eosinophils was imaged during chemotaxis by monitoring fluorescence of the calcium-sensitive dye Fura-2 with a modified digital imaging microscope. Chemotactic stimuli caused [Ca2+]i to increase in a nonuniform manner that was related to cell activity. In cells moving persistently in one direction, [Ca2+]i was highest at the rear and lowest at the front of the cell. Before cells turned, [Ca2+]i transiently increased. The region of the cell that became the new leading edge had the lowest [Ca2+]i. These changes in [Ca2+]i provide a basis for understanding the organization and local activity of cytoskeletal proteins thought to underlie the directed migration of many cells.


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The S100A4 Metastasis Factor Regulates Cellular Motility via a Direct Interaction with Myosin-IIA..
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Ca2+ Influx through L-type Ca2+ Channels Controls the Trailing Tail Contraction in Growth Factor-induced Fibroblast Cell Migration.
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Am J Physiol Renal Physiol 280, F739-F747
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J. Cell Sci. 114, 4185-4195
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Regulation of Human Eosinophil Migration Across Lung Epithelial Monolayers by Distinct Calcium Signaling Mechanisms in the Two Cell Types.
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Science 265, 1439-1441
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Microtubules are required in amoeba chemotaxis for preferential stabilization of appropriate pseudopods.
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On the crawling of animal cells.
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