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Science 25 October 1991:
Vol. 254. no. 5031, pp. 579 - 580
DOI: 10.1126/science.1948036
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Articles

Science, Vol 254, Issue 5031, 579-580
Copyright © 1991 by American Association for the Advancement of Science

articles

Cloning of a serotonin transporter affected by antidepressants

BJ Hoffman, E Mezey, and MJ Brownstein

Laboratory of Cell Biology, National Institute of Mental Health, Bethesda, MD 20892.

A complementary DNA clone for a serotonin (5HT) transporter has been isolated from rat basophilic leukemia cells. The complementary DNA sequence predicts a 653-amino acid protein with 12 to 13 putative transmembrane domains. The 5HT transporter has significant homology to the gamma-aminobutyric acid, dopamine, and norepinephrine transporters. Uptake by CV-1 cells expressing the transporter complementary DNA resembles 5HT uptake by platelets and brain synaptosomes; it is sensitive to antidepressants, amphetamine derivatives, and cocaine.


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Comparison of the Pharmacological Properties of Cloned Rat, Human, and Bovine Norepinephrine Transporters.
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Phosphorylation and Sequestration of Serotonin Transporters Differentially Modulated by Psychostimulants.
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Phosphorylation and Regulation of Antidepressant-sensitive Serotonin Transporters.
S. Ramamoorthy, E. Giovanetti, Y. Qian, and R. D. Blakely (1998)
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Review : Serotonin Transporter and Psychiatric Disorders: Listening to the Gene.
K.-P. Lesch (1998)
Neuroscientist 4, 25-34
   Abstract »    PDF »
The serotonin transporter: a primary target for antidepressant drugs.
P. Schloss and D. C. Williams (1998)
J Psychopharmacol 12, 115-121
   Abstract »    PDF »
A single serine residue controls the cation dependence of substrate transport by the rat serotonin transporter.
C. Sur, H. Betz, and P. Schloss (1997)
PNAS 94, 7639-7644
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H+ Permeation and pH Regulation at a Mammalian Serotonin Transporter.
Y. Cao, S. Mager, and H. A. Lester (1997)
J. Neurosci. 17, 2257-2266
   Abstract »    Full Text »    PDF »
Blockage of 5HT2C serotonin receptors by fluoxetine (Prozac).
Y. G. Ni and R. Miledi (1997)
PNAS 94, 2036-2040
   Abstract »    Full Text »    PDF »
Pharmacology and Distribution of Norepinephrine Transporters in the Human Locus Coeruleus and Raphe Nuclei.
G. A. Ordway, C. A. Stockmeier, G. W. Cason, and V. Klimek (1997)
J. Neurosci. 17, 1710-1719
   Abstract »    Full Text »    PDF »
Protein Kinase C Activation Regulates Human Serotonin Transporters in HEK-293 Cells via Altered Cell Surface Expression.
Y. Qian, A. Galli, S. Ramamoorthy, S. Risso, L. J. DeFelice, and R. D. Blakely (1997)
J. Neurosci. 17, 45-57
   Abstract »    Full Text »    PDF »
The Dopamine Transporter Carboxyl-terminal Tail. TRUNCATION/SUBSTITUTION MUTANTS SELECTIVELY CONFER HIGH AFFINITY DOPAMINE UPTAKE WHILE ATTENUATING RECOGNITION OF THE LIGAND BINDING DOMAIN.
F. J.S. Lee, Z. B. Pristupa, B. J. Ciliax, A. I. Levey, and H. B. Niznik (1996)
J. Biol. Chem. 271, 20885-20894
   Abstract »    Full Text »    PDF »
Ion Coupling Stoichiometry for the Norepinephrine Transporter in Membrane Vesicles from Stably Transfected Cells.
H. H. Gu, S. Wall, and G. Rudnick (1996)
J. Biol. Chem. 271, 6911-6916
   Abstract »    Full Text »    PDF »
Distinct subpopulations of enteric neuronal progenitors defined by time of development, sympathoadrenal lineage markers and Mash-1-dependence.
E Blaugrund, T. Pham, V. Tennyson, L Lo, L Sommer, D. Anderson, and M. Gershon (1996)
Development 122, 309-320
   Abstract »    PDF »
Monoamine Transporters: Basic Biology with Clinical Implications.
S. G. Amara (1995)
Neuroscientist 1, 259-267
   Abstract »    PDF »
The Role of N-Glycosylation in the Targeting and Activity of the GLYT1 Glycine Transporter.
L. Olivares, C. Aragón, C. Giménez, and F. Zafra (1995)
J. Biol. Chem. 270, 9437-9442
   Abstract »    Full Text »    PDF »
Determination of Residues in the Norepinephrine Transporter That Are Critical for Tricyclic Antidepressant Affinity.
C. Roubert, P. J. Cox, M. Bruss, M. Hamon, H. Bonisch, and B. Giros (2001)
J. Biol. Chem. 276, 8254-8260
   Abstract »    Full Text »    PDF »
A Lithium-induced Conformational Change in Serotonin Transporter Alters Cocaine Binding, Ion Conductance, and Reactivity of Cys-109.
Y. G. Ni, J.-G. Chen, A. Androutsellis-Theotokis, C.-J. Huang, E. Moczydlowski, and G. Rudnick (2001)
J. Biol. Chem. 276, 30942-30947
   Abstract »    Full Text »    PDF »
Psychostimulants Differentially Regulate Serotonin Transporter Expression in Thalamocortical Neurons.
T. L. Whitworth, L. C. Herndon, and M. W. Quick (2002)
J. Neurosci. 22, RC192
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)