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Science 25 October 1991:
Vol. 254. no. 5031, pp. 576 - 578
DOI: 10.1126/science.1948034
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Articles
Science, Vol 254, Issue 5031, 576-578
Copyright © 1991 by American Association for the Advancement of Science
Cloning and expression of a cocaine-sensitive dopamine transporter complementary DNA
S Shimada,
S Kitayama,
CL Lin,
A Patel,
E Nanthakumar,
P Gregor,
M Kuhar,
and
G Uhl
Laboratory of Molecular Neurobiology, National Institute on Drug Abuse, Baltimore, MD 21224.
A rat dopamine (DA) transporter complementary DNA has been isolated with combined complementary DNA homology and expression approaches. The DA transporter is a 619-amino acid protein with 12 hydrophobic putative membrane-spanning domains and homology to the norepinephrine and gamma-aminobutyric acid transporters. The expressed complementary DNA confers transport of [3H]DA in Xenopus oocytes and in COS cells. Binding of the cocaine analog [3H]CFT ([3H]2 beta-carbomethoxy-3 beta-(4-fluorophenyl)tropane) to transfected COS cell membranes yields a pharmacological profile similar to that in striatal membranes.
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