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Science 25 October 1991:
Vol. 254. no. 5031, pp. 568 - 571
DOI: 10.1126/science.1719633
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Articles
Science, Vol 254, Issue 5031, 568-571
Copyright © 1991 by American Association for the Advancement of Science
Phosphorylation of c-Src on tyrosine 527 by another protein tyrosine kinase
JE Thomas,
P Soriano,
and
JS Brugge
Howard Hughes Medical Institute, Department of Microbiology, University of Pennsylvania, School of Medicine, Philadelphia 19104.
The protein tyrosine kinase activity of the cellular Src protein is negatively regulated by phosphorylation at tyrosine residue 527 (Tyr527). It has not been established whether this regulatory modification of Src is mediated by autophosphorylation or by another cellular protein kinase. The phosphorylation of a modified form of c-Src that lacks kinase activity was examined in mouse cells that do not express endogenous Src (because of the targeted disruption of both src alleles). Phosphorylation of the inactive form of Src on Tyr527 occurred to a similar extent in cells lacking endogenous Src as it did in cells expressing Src. Therefore, Tyr527 phosphorylation, and thus negative control of Src kinase activity, is mediated by another cellular protein tyrosine kinase.
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