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Science 25 October 1991:
Vol. 254. no. 5031, pp. 539 - 544
DOI: 10.1126/science.1948029

Articles

Science, Vol 254, Issue 5031, 539-544
Copyright © 1991 by American Association for the Advancement of Science


articles

X-ray structure of the GCN4 leucine zipper, a two-stranded, parallel coiled coil

EK O'Shea, JD Klemm, PS Kim, and T Alber

Howard Hughes Medical Institute, Cambridge, MA 02142.

The x-ray crystal structure of a peptide corresponding to the leucine zipper of the yeast transcriptional activator GCN4 has been determined at 1.8 angstrom resolution. The peptide forms a parallel, two-stranded coiled coil of alpha helices packed as in the "knobs-into-holes" model proposed by Crick in 1953. Contacts between the helices include ion pairs and an extensive hydrophobic interface that contains a distinctive hydrogen bond. The conserved leucines, like the residues in the alternate hydrophobic repeat, make side-to-side interactions (as in a handshake) in every other layer of the dimer interface. The crystal structure of the GCN4 leucine zipper suggests a key role for the leucine repeat, but also shows how other features of the coiled coil contribute to dimer formation.


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Characterization of the Dimerization Domain in the FNR Transcription Factor.
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The crystal structure of modified bovine fibrinogen.
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J. Biol. Chem. 273, 19722-19728
   Abstract »    Full Text »    PDF »
The Human T-cell Leukemia Virus-1 Transcriptional Activator Tax Enhances cAMP-responsive Element-binding Protein (CREB) Binding Activity through Interactions with the DNA Minor Groove.
J. R. Lundblad, R. P. S. Kwok, M. E. Laurance, M. S. Huang, J. P. Richards, R. G. Brennan, and R. H. Goodman (1998)
J. Biol. Chem. 273, 19251-19259
   Abstract »    Full Text »    PDF »
Role of the PAS Domain in Regulation of Dimerization and DNA Binding Specificity of the Dioxin Receptor.
I. Pongratz, C. Antonsson, M. L. Whitelaw, and L. Poellinger (1998)
Mol. Cell. Biol. 18, 4079-4088
   Abstract »    Full Text »
Tenascin-C Hexabrachion Assembly Is a Sequential Two-step Process Initiated by Coiled-coil alpha -Helices.
R. A. Kammerer, T. Schulthess, R. Landwehr, A. Lustig, D. Fischer, and J. Engel (1998)
J. Biol. Chem. 273, 10602-10608
   Abstract »    Full Text »    PDF »
A Leucine Zipper-Like Domain Is Essential for Dimerization and Encapsidation of Bluetongue Virus Nucleocapsid Protein VP4.
N. Ramadevi, J. Rodriguez, and P. Roy (1998)
J. Virol. 72, 2983-2990
   Abstract »    Full Text »    PDF »
Neurofilament (NF) Assembly; Divergent Characteristics of Human and Rodent NF-L Subunits.
J. Carter, A. Gragerov, K. Konvicka, G. Elder, H. Weinstein, and R. A. Lazzarini (1998)
J. Biol. Chem. 273, 5101-5108
   Abstract »    Full Text »    PDF »
A Dominant-Negative Inhibitor of CREB Reveals that It Is a General Mediator of Stimulus-Dependent Transcription of c-fos.
S. Ahn, M. Olive, S. Aggarwal, D. Krylov, D. D. Ginty, and C. Vinson (1998)
Mol. Cell. Biol. 18, 967-977
   Abstract »    Full Text »



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