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Science 18 October 1991:
Vol. 254. no. 5030, pp. 439 - 441
DOI: 10.1126/science.1925604
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Articles

Science, Vol 254, Issue 5030, 439-441
Copyright © 1991 by American Association for the Advancement of Science

articles

A 32-kD GTP-binding protein associated with the CD4-p56lck and CD8-p56lck T cell receptor complexes

JC Telfer and CE Rudd

Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, MA.

The guanosine triphosphate (GTP)-binding proteins include signal-transducing heterotrimeric G proteins (for example, Gs, Gi), smaller GTP-binding proteins that function in protein sorting, and the oncogenic protein p21ras. The T cell receptor complexes CD4-p56lck and CD8-p56lck were found to include a 32- to 33-kilodalton phosphoprotein (p32) that was recognized by an antiserum to a consensus GTP-binding region in G proteins. Immunoprecipitated CD4 and CD8 complexes bound GTP and hydrolyzed it to guanosine diphosphate (GDP). The p32 protein was covalently linked to [alpha-32P]GTP by ultraviolet photoaffinity labeling. These results demonstrate an interaction between T cell receptor complexes and an intracellular GTP-binding protein.


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